| 摘要: |
| 【目的】建立光皮桦叶片高效再生体系，为光皮桦的良种培育及遗传转化研究提供依据。【方法】以光皮桦无菌叶片为外殖体，分别用含0.6 mg/L TDZ 的MS、WPM和1/2MS培养基进行不定芽诱导分化，筛选最佳基本培养基；向筛选出的最佳基本培养基中添加0.05 mg/L NAA及不同质量浓度（0.1，0.2，0.4，0.6，0.8，1.0，1.2 mg/L）TDZ的激素组合组成不定芽分化培养基，用于诱导不定芽分化，筛选最佳不定芽诱导分化培养基；以1/2MS培养基为基本培养基，分别附加0，0.05，0.1，0.5，1.0，2.0 mg/L的IBA或0.5，1.0，2.0，3.0 mg/L的NAA组成生根培养基，用于再生苗生根，筛选最佳生根培养基，建立光皮桦叶片再生体系。【结果】光皮桦无菌叶片诱导不定芽的最适基本培养基为MS培养基，其诱导的不定芽最多，且芽生长状况良好，颜色深绿；最适不定芽诱导分化培养基为：MS+TDZ 1.0 mg/L+NAA 0.05 mg/L+蔗糖30 g/L+琼脂5.5 g/L，在该培养基上叶片丛生芽诱导率可达到80.00%，平均不定芽数高达12.00个，且芽生长健壮，呈深绿色；最适再生苗生根培养基为：1/2MS+IBA 1.0 mg/L+蔗糖20 g/L+琼脂5.5 g/L，在该培养基上再生苗的生根率达100%，平均根数为14.5个，平均根长为11 cm，根较粗壮，基部无愈伤组织，且产生了很多毛细根。【结论】建立了光皮桦无菌苗叶片的高效再生体系。 |
| 关键词: 光皮桦 叶片外植体 再生体系 |
| DOI： |
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| 基金项目:国家自然科学基金项目（30600484）；浙江省科技计划项目（2008C002-1，Y200907683） |
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| Establishment of leaf-explants regeneration system of Betula luminifera H.Winkl |
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| Abstract: |
| 【Objective】The regeneration system of Betula luminifera leaf-explants was established and provide scientific reference for the gene transformation and culture of Betula luminifera.【Method】The optimal basic medium with MS,WPM and 1/2MS mediums containing 0.6 mg/L TDZ was screened using B.luminifera leaf-explants and the optimal basic medium of shoot differentiation with 0.05 mg/L NAA and TDZ of different concentrations (0.1,0.2,0.4,0.6,0.8,1.0,1.2 mg/L).Take 1/2MS as basic medium,different concentration IBA(0,0.05,0.1,0.5,1.0,2.0 mg/L) or NAA (0.5,1.0,2.0,3.0 mg/L) was added to screen the optimal root differentiation medium.The regeneration system of B.luminifera leaf-explants was established.【Result】The buds grew well with,dark green on MS medium.The suitable differentiation medium was MS+TDZ 1.0 mg/L+NAA 0.05 mg/L+Suc.30 g/L+Agar 5.5 g/L,on which the inducing rate of the adventitious shoot was 80.00% and the average buds were 12.00,and the buds grew well,dark green.The inducing rate of the root was 100%,the average roots 14.5,and the length 11 cm,the root stout,base without callus,and produced a lot of hair root when the adventitious shoots were cultured on medium of 1/2MS+IBA 1.0 mg/L+ Suc.20 g/L+Agar 5.5 g/L.【Conclusion】The regeneration system of B.luminifera leaf-explants was established. |
| Key words: Betula luminifera H.Winkl leaf-explants regeneration system |
