摘要: |
【目的】探索一种快速扩繁南瓜双单倍体植株的技术,以扩大双单倍体植株的数量,提高其育种效率。【方法】以双单倍体南瓜后代子叶为外植体,在MS培养基中分别添加不同质量浓度的6-BA(1.0,4.0 mg/L)和NAA(0.05,0.1,0.2,0.5,1.0 mg/L),筛选最佳组合;在此基础上,继续添加不同质量浓度的2,4-D(0.5,1.0,1.5,2.0 mg/L),筛选3种激素的最佳组合。在MS+6-BA 4.0 mg/L+NAA 0.2 mg/L的不定芽诱导培养基上,研究其对不同苗龄子叶的诱导效果。将不定芽接种在含不同质量浓度NAA (0.05, 0.1, 0.2, 0.5 mg/L)的MS生根培养基上,比较生根率和根系生长势。【结果】在诱芽培养基中,添加2种激素时,以6-BA 4.0 mg/L+NAA 0.2 mg/L的MS培养基诱导率最高,为66.67%,分化系数为3.89;添加3种激素时,以6-BA 4.0 mg/L+NAA 0.2 mg/L+2,4-D 1.5 mg/L的MS培养基诱导率最高,达80.95%,分化系数为4.12。当子叶展开后苗龄为3 d时,不定芽诱导率相对较大,为77.42%。不定芽在MS+0.1 mg/L NAA培养基上生根效果最好,根系生长健壮,生根率达100%。【结论】双单倍体南瓜后代子叶离体再生的适宜激素质量浓度组合为MS+6-BA 4.0 mg/L+NAA 0.2 mg/L+2,4-D 1.5 mg/L;以展开后3 d苗龄的子叶作为外植体诱导效果最好;MS+0.1 mg/L NAA是不定芽诱导生根的最适培养基。 |
关键词: 南瓜 双单倍体植株 子叶 离体培养 植株再生 |
DOI: |
分类号: |
基金项目:国家大宗蔬菜产业技术体系项目(CARS-25);国家“十一五”科技支撑计划项目(2009BAD8B02) |
|
Study on in vitro regeneration of double haploid plants offspring of pumpkin(Cucurbita moschata Duch.) with cotyledon explants |
|
Abstract: |
【Objective】We explored a highly efficient plantlet regeneration technology on double haploid plants of pumpkin to get a great quantity of double haploid plants to promote the efficiency in pumpkin breeding.【Method】Taking the cotyledon of double haploid offspring of pumpkin as the explants,we inoculated on several types of MS-based media which were added 6-BA (1.0,4.0 mg/L) and NAA (0.05,0.1,0.2,0.5,1.0 mg/L) in different concentrations to select the optimal concentration combination;Based on this,we continued to study adding different concentrations of 2,4-D (0.5,1.0,1.5,2.0 mg/L),and screen three hormone optimal combination.The effect of the different ages of cotyledon explants in MS+6-BA 4.0 mg/L+NAA 0.2 mg/L adventitious bud inductive medium was studied.Adventitious buds were inoculated on MS medium supplement with different concentrations (0.05,0.1,0.2,0.5 mg/L) of NAA.The condition of rooting rate and growth vigour of root was analyzed.【Result】In the bud induction medium,adding two hormones MS+6-BA 4.0 mg/L+NAA 0.2 mg/L induced the highest rate 66.67%,and the differentiation coefficient was 3.89;Adding three kinds of hormones MS+6-BA 4.0 mg/L+NAA 0.2 mg/L+2,4-D 1.5 mg/L induced the highest rate of 80.95%,differentiation coefficient was 4.12.Adventitious bud induction rate was bigger,77.42%,when the age of cotyledon was 3 days after unfolding.The effect of adding 0.1 mg/L NAA in MS medium on rooting culture was better,the root can grow strongly,rooting rate of adventitious bud reached 100%.【Conclusion】The suitable hormone concentration combination for in vitro regeneration of the cotyledon of double haploid plants offspring is MS+6-BA 4.0 mg/L+NAA 0.2 mg/L+2,4-D 1.5 mg/L.3-day-old cotyledon after unfolding as the explants expresses the best induction effect.MS+0.1 mg/L NAA is the suitable medium for root induction. |
Key words: pumpkin double haploid plant cotyledon in vitro culture plant regeneration |