| 摘要: |
| 【目的】对美洲棘蓟马体内共生菌Wolbachia进行分子生物学鉴定，确定该蓟马体内Wolbachia的进化位置，为进一步探讨Wolbachia对其生殖作用的调控机制提供理论依据。【方法】以wsp基因为目的基因，对美洲棘蓟马体内的共生菌Wolbachia进行特异性扩增和测序，使用Clustal X 1.83软件对所得DNA序列进行比对；在MEGA 4.0软件中采用邻接法对Wolbachia的系统发育关系进行分析。【结果】利用wsp基因的特异性引物从美洲棘蓟马体内扩增出了632 bp的Wolbachia的wsp基因片段(GenBank登录号为JN315668)，580 bp的Wolbachia A群的wsp基因片段和405 bp的Mel亚群的wsp基因片段。系统发育分析结果表明，美洲棘蓟马体内的Wolbachia与黑腹果蝇亲缘关系较近。【结论】美洲棘蓟马体内感染的Wolbachia属于A群Mel亚群。 |
| 关键词: 美洲棘蓟马 Wolbachia wsp 序列分析 系统发育 |
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| 基金项目:高等学校博士点专项科研基金项目(20090204110003)；科技部基础性工作专项(2006FY120100) |
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| Sequencing and phylogenetic analysis of the wsp gene of Wolbachia in Echinothrips americanus Morgan |
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| Abstract: |
| 【Objective】 The purpose of this study is to identify the presence of Wolbachia in Echinothrips americanus Morgan,1913 (Thysanoptera:Thripidae) based on the amplification and sequencing of the wsp gene and definite the phylogenetic relationship of Wolbachia in this host.The result of this study will provide theoretic basis for the function of Wolbachia on manipulation of the reproductive biology in E.americanus.【Method】 In this study,the phylogenetic relationships of Wolbachia were analyzed by using wsp sequences.All wsp sequences were aligned using the ClustalX 1.83 program.Phy1ogenetic tree was constructed with neighbor joining implementation.【Result】 Using the specific primers,632 bp region of Wolbachia wsp gene(GenBank accesstion number is JN315668),580 bp region of Wolbachia group A wsp gene and 405 bp region of Wolbachia Mel subgroup wsp gene were sequenced from this host population.【Conclusion】 The E.americanus was infected with endosymbiont Wolbachia.The topology of the phylogenetic tree showed that the Wolbachia in E.americanus belonged to group A. |
| Key words: Echinothrips americanus Wolbachia wsp sequence analysis molecular phylogeny |
