| 摘要: |
| 【目的】研究中华蟾蜍(Bufo gargarizans)蝌蚪在不同发育阶段甲状腺的组织学变化。【方法】以处于变态高峰前后5期（28,33,38,42,46期）的中华蟾蜍蝌蚪为试验材料，用Motic BA300光学显微镜对各个发育时期蝌蚪的甲状腺进行组织学观察，测定蝌蚪的全长、体质量和肝质量等形态指标，并分析这些形态指标与甲状腺直径、滤泡平均直径及最大直径的线性相关性。【结果】在28期时，中华蟾蜍蝌蚪甲状腺出现但无分泌甲状腺激素的功能；33期时，甲状腺具备了分泌甲状腺激素的能力；此后甲状腺进一步发育，在蝌蚪前肢展开期甲状腺活性达到最强；至尾完全吸收期，甲状腺机能减弱。蝌蚪全长、体质量及肝质量与甲状腺直径及滤泡直径呈正线性相关。【结论】具有分泌机能的甲状腺出现的发育时期，与两栖动物变态类型相关；分泌高峰期出现的发育时期与幼体持续时间密切相关。中华蟾蜍蝌蚪的全长可以直观地反映其甲状腺的机能活性。 |
| 关键词: 中华蟾蜍 蝌蚪 甲状腺 |
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| 基金项目:中央高校基本科研业务费专项（GK261001） |
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| Changes of thyroid gland histological structures during the metamorphosis of Bufo gargarizans tadpole |
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| Abstract: |
| 【Objective】The research was to study the changes of thyroid gland histological structures in tadpole of Bufo gargarizans during development.【Method】Tadpoles were taken from every five stages before and after metamorphosis(stage 28,stage 33,stage 38,stage 42 and stage 46),and histological structures of thyroid glands in tadpoles were observed by Motic BA300 microscope.In addition,total length,body weight and liver weight of the tadpoles were measured.Then,the relationship was analyzed between total length,body weight,liver weight and thyroid gland,follicle diameter and follicle number.【Result】The thyroid gland was first present at the stage 28,but it lacked function.Thyroid gland possessed the ability of secreting hormone at the stage 33.The activity of thyroid gland reached peak when forelimbs unfolded.The activity of thyroid gland declined,when the tail began to dissolve.There was a linear correlation between total length,body weight,liver weight and thyroid gland diameter and follicle diameter.【Conclusion】The developmental stage in which the thyroid gland begins to secrete thyroid hormone is related with the amphibian metamorphic types;and the larval period is related to the developmental stage,at which the thyroid gland reaches peak.Changes of body length in B.gargarizans tadpole may reflect the activity of the thyroid gland directly. |
| Key words: Bufo gargarizans tadpole thyroid gland |
