| 摘要: |
| 【目的】观察猪圆环病毒2型（PCV-2）感染对猪肾传代细胞（PK-15细胞）炎性细胞因子白细胞介素（IL） mRNA转录水平的影响，探讨宿主与病毒之间的作用关系及细胞炎性反应机制。【方法】以未感染PCV-2的PK-15细胞为对照组，运用相对定量PCR技术，测定和分析PCV 2感染PK 15细胞后，PCV-2 DNA相对含量的变化，以及炎性细胞因子IL-6、IL-8、IL-12p35、IL-12p40、IL-13、IL-17、IL-18 mRNA转录水平在1，6，12，24，48，和72 h的变化。【结果】PCV-2感染后，PK-15细胞的IL-6、IL-13、IL-17、IL-18 的mRNA转录水平在12 h显著增加，24 h后mRNA转录水平下降；IL-8的mRNA转录水平在48 h时最高，为对照组的2.5倍，但72 h时恢复至与对照组水平相当；随着感染时间的延长，IL-12p35、IL-12p40 的mRNA转录水平显著下降。【结论】PCV-2感染后可引起PK-15细胞中IL-6、IL-8、IL-13、IL-17、IL-18等细胞因子mRNA转录水平增加，而IL-12 mRNA转录水平下降，提示PCV-2感染后引起的PK-15细胞炎性反应与其分泌的炎性细胞因子的改变有关。 |
| 关键词: PK-15细胞 炎性细胞因子 白细胞介素 mRNA转录水平 猪圆环病毒2型 |
| DOI： |
| 分类号: |
| 基金项目:河南省杰出人才创新基金项目（0621002100） |
|
| Analysis of the transcriptional profiles of inflammatory related cytokines of PK-15 cell cultures following Porcine circovirus type 2 infection |
|
SHANG Yanhong, LIU Xinxin, LI Jinlei,et al
|
|
Key Laboratory for Animal-derived Food Safety of Henan Province,et al
|
| Abstract: |
| 【Objective】The study was conducted to investigate the effect of Porcine circovirus type 2 (PCV-2) on the expression of inflammatory cytokines in PK-15 cells (PK-15),and to explore the host and PCV-2 interaction and the mechanism for cellular inflammatory responses.【Method】PCV-2 was used to infect PK-15 cells,then the viral DNA and the transcript level of inflammatory cytokines (IL-6,IL-8,IL-12p35,IL-12p40,IL-13,IL-17 and IL-18) were detected and analyzed in different post-infectious time using real-time PCR,respectively.【Result】The results showed that the mRNA transcript levels of IL-6,IL-13,IL-17,IL-18 significantly decreased on 12 h postinoculation (p.i.),however,the mRNA transcript levels notably increased on 24 h p.i.The mRNA transcript levels of IL-8 were 2.5 times the control group within 48 h p.i,and notably increased on 24 h p.i;The mRNA transcript levels of IL-12p35,IL-12p40 were significantly lower than the control with time.【Conclusion】The mRNA transcript levels of IL-6,IL-8,IL-13,IL-17,IL-18 cytokines increased,and the level of IL-12 decreased when PCV-2 infected PK 15 cells.The results suggested that the inflammatory responses of PK-15 cells induced by PCV-2 infection were associated with the changes of inflammatory related cytokines. |
| Key words: PK-15 cell inflammatory related cytokines IL transcriptional profiles of mRNA porcine circovirus type 2 |
