| 摘要: |
| 【目的】研究山萘根茎提取物及其萃取物对南方根结线虫生长发育的影响，为南方根结线虫的防治提供支持。【方法】以室内培养和盆栽试验的方法，研究了山萘根茎提取物以及其不同萃取物对南方根结线虫胚胎发育、胚后发育和2龄幼虫孵化的影响。【结果】1 000 mg/L山萘正己烷萃取物、氯仿萃取物对南方根结线虫的胚胎发育和卵的孵化具有明显的抑制作用，2种萃取物对南方根结线虫2龄幼虫3，6，9 d的孵化抑制率均为100%；山萘根茎甲醇粗提物对南方根结线虫胚后发育的影响，主要表现在4龄幼虫发育为成虫的发育历期推迟了3 d，雌虫产卵的初产日推迟了2 d，并且雌虫产卵量减少。【结论】山萘根茎提取物对南方根结线虫生长发育具有明显的抑制作用。 |
| 关键词: 山萘 南方根结线虫 胚胎发育 胚后发育 |
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| 基金项目:国家自然科学基金项目(30400037) |
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| Effect of Kaempferia galanga L.on the development of Meloidogyne incongnita |
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| Abstract: |
| 【Objective】The objective of this paper was to study the effect of the extract of Kaempferia galanga L. on the development of root-knot nematode,Meloidogyne incongnita.【Method】The effects on embryonic development, postembryonic development as well as the hatching of second instar larvae of root-knot nematode,M.incongnita were studied by indoor culture and pot experiment.【Result】The results showed that the n-hexane extracts and the chloroform extracts could significantly inhibit embryonic development and larva hatching,the inhibition rate of hatching of the two extracts to second instar larvae on the 3rd,6th,9th day reached 100%;The pot experiments revealed that the development of post-embryonic from fourth instar larvae to adult was prolonged 3 days longer when treated with the methanol extracts of K.galanga L.,while the the female reproduction was prolonged 2 days longer and the egg laying number was reduced significantly. 【Conclusion】The extracts of K.galanga L. could significantly inhibit the development of root-knot nematode,M.incongnita,providing theoretical basis for applying K.galanga L.to control plant nematode diseases. |
| Key words: Kaempferia galanga L. Meloidogyne incongnita development of embryonic development of post-embryonic |
