| 摘要: |
| 【目的】分析寄生疫霉菌侵染胁迫下拟南芥表达的microRNA，为探明microRNA对应靶标基因的调控网络及其可能的病理学和生物学功能奠定基础。 【方法】以寄生疫霉菌（Phytophthora parasitica）和模式植物拟南芥（Arabidopsis thaliana）为材料，鉴定在病菌侵染胁迫下拟南芥表达的microRNA，构建其靶标调控网络，并进行Gene Ontology和蛋白质结构域富集分析。 【结果】在寄生疫霉菌侵染拟南芥的Solexa测序文库中鉴定39条拟南芥microRNA，筛选出了5个转录因子相关的调控网络；Gene Ontology富集结果表明，在生物学过程中，转录调控相关的基因显著富集；蛋白质结构域富集结果表明，靶基因中转录因子显著富集。 【结论】拟南芥响应寄生疫霉菌microRNA靶标基因的功能主要集中在转录因子的调控功能上。 |
| 关键词: MicroRNA 生物胁迫 调控网络 GO分析 |
| DOI： |
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| 基金项目:国家“863”计划项目（2008AA02Z110） |
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| Analysis of the regulation network of microRNA targets in Arabidopsis thaliana infected with Phytophthora parasitica |
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ZHANG Wei, XU Ke, WANG Qinhu
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College of Life Sciences, Northwest A&F University
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| Abstract: |
| 【Objective】We analyzed the microRNAs of Arabidopsis in response to infection by Phytophthora parasitica,to understand microRNA targets regulation network,potential pathological and biological roles in processes of response to challenged by infection.【Method】The compatible Phytophthora parasitica Arabidopsis thaliana pathosystem was employed to identify plant microRNAs in response to pathogen infection,to construct the target genes regulation networks,and to perform Gene Ontology and domain enrichment analysis.【Result】By Solexa sequencing,a total of 39 microRNAs were identified from Arabidopsis infected with P.parasitica,of which five transcription factors regulated networks were identified.Gene Ontology annotation indicated that the transcription regulation associated genes were significantly enriched;protein domain annotation showed that transcription factors were significantly enriched in the target genes.【Conclusion】The results show that the function of microRNA targets in Arabidopsis in response to infection by P.parasitica was enriched for regulation of transcription factors. |
| Key words: microRNA biotic stress regulation network GO analysis |
