| 摘要: |
| 【目的】研究雌激素与γ-干扰素(IFN-γ)对下丘脑的协同保护作用。【方法】建立去卵巢（OVX）模型大鼠，然后将其分为摘除卵巢（OVX）组、假手术（SHAM）组、OVX+17-β雌二醇(E2)组、OVX+E2+50 IU IFN-γ组、OVX+E2+200 IU IFN-γ组和OVX+E2+800 IU IFN-γ组共6组，分别在处理后的0，2，4，6，8周，用免疫组织化学SP法对各组大鼠下丘脑中凋亡相关蛋白Bcl-2和Bax的表达进行检测。【结果】OVX组大鼠下丘脑中Bcl-2的相对表达量随时程逐渐减少，4周后最低，6周后又逐渐上升，随后维持在一定的水平，Bax表达量的变化趋势则与之相反；OVX组Bcl-2和Bax的表达量与SHAM组差异极显著(P＜0.01)。OVX+E2组大鼠下丘脑中Bcl-2的相对表达量增加，Bax的相对表达量降低，均与OVX组差异显著(P＜0.05)。OVX＋E2+200 IU IFN-γ组Bcl-2的相对表达量极显著高于OVX组，显著高于OVX+E2组，Bax的总体变化趋势与OVX+E2组一致；OVX+E2+50 IU IFN-γ组和OVX+E2+800 IU IFN-γ组Bcl-2和Bax的相对表达量与OVX+E2组差异不显著(P＞0.05)。【结论】雌激素的缺乏增强了下丘脑神经元对凋亡的敏感性，而外源生理剂量E2与适当剂量的IFN-γ，在一定时期内对这种凋亡有重要的保护作用。 |
| 关键词: 去卵巢大鼠 Bcl-2/Bax 雌激素 γ-干扰素 下丘脑 |
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| 基金项目:陕西省自然科学基金项目(SJ08C105)；抗病转基因牛新品种培育重点项目(2009ZX08007-008B) |
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| Effect of estrogen and IFN-γ on the expression of Bcl-2 and Bax in hypothalamus of ovariectomed rats |
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| Abstract: |
| 【Objective】The research was to study the synergy effect of estrogen and interferon-γ(INF-γ) on protecting the hypothalamus nucleus.【Method】The immunohistochemical SP method was used to study the expressions of Bcl-2 and Bax in hypothalamus of the ovariectomized (OVX) group,the SHAM group,the OVX+17-β estrogen(E2) group,OVX+E2+50 IU IFN-γ group,OVX+E2+200 IU IFN-γ group and OVX+E2+800 IU IFN-γ group.【Result】The main results were as follows:After ovariectomization,relative expressions of Bcl-2 positive productions(μ2) tapered with the course and reached the minimum after 4 weeks concurrently.The μ2 of Bax increased and reached the maximum after 4 weeks,which had significant difference with the SHAM group(P＜0.01).OVX+E2 group,the μ2 of Bcl-2 increased and had remarkable difference with OVX group(P＜0.01),the μ2 of Bax decreased and had remarkable difference with OVX group(P＜0.01);The μ2 of Bcl-2 of OVX+E2+200 IU IFN-γ group had different changes with OVX group(P＜0.01),OVX+E2 group(P＜0.05),and SHAM group(P＞0.05).The total tendency of the μ2 of Bax had very significant difference with OVX group(P＜0.01) in the nucleus which the μ2 of Bax decreased.【Conclusion】The sensibility of the neurons in hypothalamus to apoptosis was enhanced by estrogen deficiency,and physiological dose E2 combined with proper-dose IFN-γ had important protecting effect on the apoptosis within a certain period. |
| Key words: ovariectomized rat Bcl-2/Bax estrogen IFN-γ hypothalamus |
