| 摘要: |
| 【目的】构建microRNA-144（miR-144）的真核表达质粒，并探讨miR-144对人肝癌细胞株HepG2生物学行为的影响，为肝癌的生物学治疗提供依据。【方法】构建miR-144真核表达质粒并合成miR-144反义寡核苷酸，通过实时定量PCR检测miR-144的表达，采用MTT比色法及流式细胞仪技术，检测抑制与过表达miR-144对肝癌细胞增殖、凋亡和细胞周期的影响。【结果】成功构建了miR-144的真核表达质粒，过表达miR-144能够显著抑制肝癌细胞的增殖（P<0.05），促进细胞凋亡（P<0.05）；而抑制miR-144的表达则得到相反的结果；抑制与过表达miR-144对肝癌细胞周期的影响都不大（P>0.05）。 【结论】miR-144能影响人肝癌细胞株HepG2的生物学行为，具有抑制肝癌细胞增殖的作用。 |
| 关键词: miR-144 表达质粒 肝癌 HepG2 生物学行为 |
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| 基金项目:国家自然科学基金项目（81071877）；西安交通大学新兴前沿、学科综合交叉项目(No2010-96) |
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| Construction of microRNA-144 plasmid and its effect on biological behavior of human hepatocellular carcinoma |
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| Abstract: |
| 【Objective】The study was to construct microRNA-144 (miR-144) eukaryotic expression vector and to detect the effect of miR-144 on the proliferation,apoptosis,migration,and invasion of human hepatocellular carcinoma HepG2 cells.【Method】After the construction of miR-144 expression plasmid and miR-144 antisense oligonucleotides,the effect of them on expression of miR-144 was confirmed by real-time PCR.The proliferation,apoptosis,migration,and invasion of HepG2 were detected by MTT assay and flow cytometry respectively.【Result】Overexpression of miR-144 could significantly inhibit the proliferation(P<0.05) and promote the apoptosis of HepG2（P<0.05）.However,inhibition of miR-144 provides the opposite results. However, alteration of miR-144 could not influence the cell cycle（P>0.05）.【Conclusion】miR-144 could influence the biological behavior of primary liver cancer cell HepG2 and acts as a tumor suppressor. |
| Key words: miR-144 expression plasmid liver cancer HepG2 biological behavior |
