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獭兔双歧杆菌的培养特性及抗菌活性研究
史怀平1, 解广勤2, 史新娥1
1.西北农林科技大学 动物科技学院;2.武警陕西省总队后勤部农副业基地
摘要:
【目的】研究獭兔双歧杆菌的生理特性及抗菌能力。【方法】将獭兔双歧杆菌接种于特定的培养基后,观察其生长特性,并与大肠埃希菌共培养,研究其抗菌能力。【结果】獭兔双歧杆菌呈分枝状,在改良肉汤培养基中生长旺盛;耐温驯化后发现,獭兔双歧杆菌可在42 ℃下生长;獭兔双歧杆菌对许多药物如红霉素等敏感;獭兔双歧杆菌与大肠埃希菌共培养可抑制大肠埃希菌生长,其代谢产物也具有明显的抑菌能力。【结论】獭兔双歧杆菌在体外培养良好,具有很好的抗菌活性。
关键词:  獭兔  双歧杆菌  抗菌活性
DOI:
分类号:
基金项目:陕西省自然科学基金项目(2010JM3016)
Culturing characteristics and antibacterial activity of Rex Rabbit bifidobacterium
Abstract:
【Objective】This research studied the physiological characteristics and antimicrobial ability of Rex Rabbit bifidobacterium.【Method】After Rex Rabbit bifidobacteria were inoculated in indicated medium,the growth characteristics were investigated.And the antimicrobial activity of Rex Rabbit bifidobacterium was detected through co-culturing with E.coli.【Result】Rex Rabbit bifidobacteria were cladodromous and significantly proliferated in improved broth.It was found through heat-resistant assay that Rex Rabbit bifidobacterium could grow at 42 ℃.Rex Rabbit bifidobacteria were sensitive to many drugs like Erythromycin.After Rex Rabbit bifidobacterium were co-cultured with E.coli,the growth of E.coli was blocked and the metabolites from Rex Rabbit bifidobacterium also had obvious antimicrobial function.【Conclusion】Rex Rabbit bifidobacteria grew well in vitro and had significant antimicrobial activity.
Key words:  Rex Rabbit  bifidobacterium  antimicrobial activity