| 摘要: |
| 【目的】对抗真菌活性菌株BP08进行鉴定，并对其最适培养条件和最佳培养基组分进行优化。【方法】从华南农业大学杀虫植物标本园土壤中筛选出1株BP08拮抗菌株，采用形态和生理生化鉴定方法、16S rDNA序列测定和系统发育分析对菌株BP08进行鉴定；以菌体生物量和发酵液拮抗水稻纹枯病菌活性为指标，通过单因素试验，对培养基初始pH值、发酵温度、装液量、接种量等进行优化，在此基础上以发酵液拮抗水稻纹枯病菌活性为指标，对发酵培养基组分进行优化。【结果】筛选的菌株BP08初步鉴定为短小芽孢杆菌；在培养基初始pH值7.0～8.0，发酵温度30 ℃，装液量100 mL/L，接种量为3%，180 r/min条件下，菌株BP08生长量及拮抗水稻纹枯病菌的活性较高。培养基最佳碳源、氮源、无机盐分别为葡萄糖、牛肉膏、CaCl2。【结论】短小芽孢杆菌菌株BP08在优化培养条件下能够得到大量的抗真菌物质，具有一定的开发利用潜力。 |
| 关键词: 抗真菌活性菌株BP08 短小芽孢杆菌 培养条件优化 培养基优化 |
| DOI： |
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| 基金项目:广州市科技局项目（2008J1-C221） |
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| Identification of antifungal bacteria strain BP08 and optimization of its fermentation conditions |
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| Abstract: |
| 【Objective】The research identified the antifungal activity bacteria strain BP08,optimized the fermentation conditions and selected the best medium components.【Method】The strain BP08 was isolated from soil of South China Agricultural University’s herbarium garden,by the physiology and homologic analysis of 16S rDNA sequence to identify the strain BP08.In terms of bacterial quantity and the anti-rhizoctonia solani activity,the initial pH,inoculation volume,medium volume,fermentation temperature were measured through the single factor experiments.On that basis,the components of fermentation medium were optimized in terms of optimization of fermentation conditions.【Result】The strain BP08 was identified as Bacillus pumilus strain.The results of optimization of fermentation conditions showed that when the medium initial pH was 7.0-8.0,the fermentation temperature 30 ℃,the quantity of medium 100 mL in a 1 000 mL flask,the inoculation volume 3%,the rotation speed about 180 r/min,the bacterial quantity and anti Rhizoctonia solani activity both were the maximum.The best carbon source,inorganic nitrogen source,and inorganic salt source respectively were glucose,beef extract and the CaCl.【Conclusion】B.pumilus strains BP08 could produce a large number of antifungal substances under normal fermentation conditions,and thus has a good production potential applications. |
| Key words: antifungal activity strain BP08 Bacillus pumilus optimization of fermentation conditions optimization of medium components |
