| 摘要: |
| 【目的】研究特定拮抗放线菌对西洋参人参土传病害病原真菌的接触抗菌机理。【方法】以5株西洋参、人参土传病害病原真菌菌体为惟一碳源，用液体培养及3，5-二硝基水杨酸（DNS）法研究5株供试病原真菌对9株拮抗放线菌几丁质酶和纤维素酶合成的诱导作用；采用搭片法，观察9株拮抗放线菌与5株供试病原真菌菌丝间的相互作用。【结果】①以5株病原真菌菌体为惟一碳源时，可诱导9株拮抗放线菌合成几丁质酶和纤维素酶；9株放线菌的几丁质酶和纤维素酶活性分别为7.17～11.58和6.14～21.20 U，其中西洋参恶疫霉菌体对9株放线菌几丁质酶、纤维素酶合成的诱导作用强于其他病原真菌；②拮抗放线菌Act11、Act13、Act24及D141的菌丝与供试病原真菌菌丝接触时，对西洋参锈腐病菌、人参锈腐病菌菌丝有明显的溶解作用。【结论】以5株供试病原真菌菌体为惟一碳源时，可诱导9株拮抗放线菌合成几丁质酶和纤维素酶；供试拮抗放线菌与西洋参病原菌接触时的抗菌作用，是通过放线菌产生几丁质酶和纤维素酶，破坏细胞壁，使病原真菌菌丝溶解的方式完成的。 |
| 关键词: 西洋参 人参 病原真菌 诱导酶 放线菌 几丁质酶 纤维素酶 |
| DOI： |
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| 基金项目:长江学者和创新团队发展计划资助项目（IRT748） |
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| Inductive effects of fungal pathogens of American Ginseng and Ginseng on chitinase and cellulase of antigonistic actinomycetes |
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| Abstract: |
| 【Objective】Inhibitory mechanism of antigonistic actimomycetes fighting against fungal pathogens of soil-borne disease in American Ginseng and Ginseng was studied.【Method】The inductiveness was assessed by the activity of chitinase and cellulase,which were induced from 9 strains of antigonistic actinomycete by using 5 dried strains of fungal pathogens of American Ginseng and Ginseng as C-source in liquid culture medium and DNS measurement,and the mutual effects were observed between antigonistic actinomycetes and fungal pathogens of American Ginseng and Ginseng through the method of building pieces on plate.【Result】①The activity of chitinase and cellulase,which was mainly distributed among 7.17-11.58 and 6.14-21.20 U,respectively,differed from 9 strains of antigonistic antinomycete induced from 5 strains of fungal pathogen of American Ginseng and Ginseng.The powder of Phytophthora cactorum could induce antigonistic actinomycetes to produce much more chitinase and cellulase.②The mutural effects between mycelia of Act11,Act13,Act24 and Cylindrocarpon destructans,Cylindrocarpon sp.,such as winding and decomposition,were observed distinctively.【Conclusion】The mycelia of 5 strains of fungal pathogen of American Ginseng and Ginseng could induce 9 strains of antigonistic actinomycete to produce chintinase and cellulase;9 strains of antigonistic actinomycete had inhibitory effects on the growth of 5 strains of fungal pathogen of American Ginseng and Ginseng by producing cell-wall degrading enzymes,such as chitinase and cellulase,which can promote mutural effects between mycelia,such as winding,decomposition. |
| Key words: American Ginseng Ginseng fungal pathogen inducible enzyme actinomycete chitinase cellulase |
