| 摘要: |
| 【目的】研究自制复方中药对肥胖模型小鼠的减肥、降脂和健脾作用。【方法】构建小鼠肥胖模型，成功后将其分为模型对照组(灌胃蒸馏水0.2 mL/(只·d))、阳性对照组(灌胃6.5 mg/mL西布曲名溶液0.2 mL/(只·d))和药物组(灌胃复方中药浸膏0.3 mL/(只·d))，同时设立普通对照组(处理方法同模型对照组)，20 d后检测分析其李氏指数、体质量增长率、脂肪系数和血脂水平等指标的变化情况，记录各组小鼠抗疲劳试验的游泳时间及其活力表现，并取肝脏组织提取总RNA，用RT-PCR方法检测脂肪酸合酶(FAS)基因的表达量。【结果】药物组李氏指数、体质量增长率、脂肪系数及血清甘油三酯、总胆固醇和高密度脂蛋白-胆固醇水平与模型对照组相比均显著降低(P＜0.05)，游泳时间明显长于3个对照组(P＜0.05)，脂肪酸合酶基因mRNA表达量高于模型对照组而低于普通对照组。【结论】供试复方中药具有显著的减肥降脂和健脾作用。 |
| 关键词: 复方中药 健脾 减肥 降血脂 脂肪酸合酶 |
| DOI： |
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| 基金项目:陕西省重大科技创新专项(K332020916) |
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| Study on functions of the compound traditional Chinese medicine in invigorating spleen and in losing weight and fat |
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| Abstract: |
| 【Objective】The research was conducted to study the functions of a homemade compound traditional Chinese medicine in reducing weight and fat and clinic effect in invigorating spleen of obesity mice.【Method】Constructed obesity model mice were divided into model control group(perfused with distilled water,0.2 mL/(mouse·day)),positive control group (perfused with 6.5 mg/mL sibutramine solution,0.2 mL/(mouse·day))and drug therapy group (perfused with the compound traditional Chinese medicine extract,0.3 mL/(mouse·day)).Meanwhile,a normal control group (treatment was the same as that of the model control group) was established.20 days later,obesity indicators,such as Lee index,growth rate of body weight,fat index,blood lipid level and etc,were calculated;swimming time was recorded in fatigue tests,and dynamic performance was observed.Meanwhile,total RNA was isolated from liver and FAS gene expression level was measured by RT-PCR.【Result】The indicators of drug therapy group,such as Lee index,growth rate of body weight,fat index,blood lipid level and etc,were significantly lower than those of modeled group (P＜0.05);Meanwhile,the swimming time of drug therapy group was longer than that of the three control groups (P＜0.05).However,FAS gene expression level of drug therapy group was between modeled group and control group.【Conclusion】The compound traditional Chinese medicine possesses certain effect in both reducing weight and fat and invigorating spleen. |
| Key words: compound traditional Chinese medicine invigorating spleen weight-loss fat reducing FAS |
