| 摘要: |
| 【目的】对秦川牛Chemerin基因第2和第6外显子进行SNPs检测，并研究其与秦川牛肉质性状的相关性。【方法】采用DNA直接测序技术并结合PCR-SSCP方法，对324头2～3岁秦川牛Chemerin基因第2和第6外显子进行多态性分析，用最小二乘法拟合线性模型对Chemerin基因不同基因型与秦川牛肉质性状(宰前活质量、胴体质量、背膘厚、大理石花纹评分、系水力、眼肌面积和嫩度)进行关联分析。【结果】秦川牛Chemerin基因第2外显子868 bp处发生了A→G突变，第6外显子2 948 bp处发生了C→G突变；用PCR-SSCP方法检测得到AA、AG、GG、EE和EF 5种基因型，其基因型频率分别为0.219 1，0.432 1，0.348 8，0.348 4和0.651 6，均处于中度多态，其中C2948G位点处于Hardy-Weinberg极度不平衡状态(P＜0.01)。关联分析结果表明，秦川牛Chemerin基因第2外显子AA基因型与眼肌面积、背膘厚、大理石花纹评分和系水力均显著相关（P＜0.05）；第6外显子各基因型与供试肉质性状间没有相关性（P＞0.05）。【结论】Chemerin基因第2外显子可能是影响秦川牛眼肌面积、系水力和大理石花纹评分等肉质性状的主效QTL或与之紧密连锁，可以尝试将其用作秦川牛品质改良的辅助选择标记。 |
| 关键词: 秦川牛 Chemerin基因 PCR-SSCP 多态性 肉质性状 |
| DOI： |
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| 基金项目:国家“863”计划项目(2006AA10Z1A1，2008AA101010，2010AA10Z101)；国家科技支撑计划项目(2006BAD01A10-3)；国家转基因重大专项(2008ZX08007-002)；教育部“长江学者和创新团队发展计划”项目(IRT0940) |
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| Polymorphism of Chemerin gene and its relationship with meat quality traits in Qinchuan cattle |
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SONG Fubiao1, ZAN Linsen2,3, WANG Hongcheng,et al1
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1.College of Animal Science and Technology, Northwest A＆F University;2.College of Animal Science and Technology Northwest A＆F University;3.National Beef Cattle Improvement Center
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| Abstract: |
| 【Objective】The variation of Exon 2 and Exon 6 of Chemerin gene were examined and the association of this polymorphism with meat quality traits in Qinchuan cattle was investigated.【Method】Using PCR-SSCP and DNA sequencing technology,324 individual Qinchuan heifers aged 2 to 3 years were selected to check the polymorphism of Exon 2 and Exon 6 of Chemerin gene.Least squares regressions analysis with linear mode was used to analyze the relationship between the genotypes and meat quality traits.【Result】Mutations of A868G and C2948G were detected in the Exon 2 and Exon 6,respectively.Five genotypes (AA,AG,GG,EE and EF) were found by PCR-SSCP.And the genotypic frequencies of them were 0.219 1,0.432 1,0.348 8,0.348 4 and 0.651 6,respectively.Both of them were in moderate diversity,while the C2948G location was not in Hardy Weinberg balance (P＜0.01).Association analysis of polymorphisms with meat quality traits showed that:the genotype AA was significantly related to the loin muscle area,marbling score and the water holding capability of Exon 2 of Chemerin gene (P＜0.05),while there was no significant association in the live weight,carcass weight and meat tenderness(P＞0.05).The results also showed that Exon 6 polymorphism had no association with any meat quality traits.It may mean that there is no correlation between the polymorphism and the meat quality traits.【Conclusion】We conclude that Chemerin gene Exon 2 may be closely related to the meat quality traits,such as loin muscle area,water holding capability and marbling score,and it could be a candidate molecular marker for the quality improved of Qinchuan cattle. |
| Key words: Qinchuan cattle Chemerin gene PCR-SSCP polymorphism meat quality traits |
