| 摘要: |
| 【目的】研究不同强度微波照射对麦长管蚜（Sitobion avenae）生态学参数及体内抗氧化酶系统的影响，为麦长管蚜种群爆发、综合治理、微波辐射产生毒性效应的分子机理及微波对昆虫种群遗传进化的影响研究提供理论依据。【方法】用不同强度微波照射（功率分别为2，4，6 W；照射时间分别为10，20，30 s）麦长管蚜后，对第2代麦长管蚜进行特定时间生命表研究，分析微波辐射对麦长管蚜生态学参数的影响，并采用非变性聚丙烯酰胺凝胶电泳（PAGE）方法，分析微波辐射对麦长管蚜体内抗氧化酶同工酶的影响。【结果】微波处理后，麦长管蚜的平均世代周期(T)、净增殖率(R0)、内禀增长率(Rm)均在中低强度（2，4 W）照射时较对照有所提高，而在高强度（6 W）照射时较对照显著降低。微波处理对麦长管蚜过氧化物酶（POD）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）等抗氧化酶系统有明显影响，POD同工酶有新酶带出现,CAT和SOD同工酶酶带亮度随着微波辐射强度的增大而减弱。【结论】微波胁迫后麦长管蚜的生态学参数发生了变化，说明微波对麦长管蚜的毒害已遗传到下一代，高强度的微波处理对其影响更大。微波胁迫对麦长管蚜POD同工酶具有明显的激活效应，但是对CAT和SOD同工酶有抑制作用。 |
| 关键词: 微波辐射 麦长管蚜 生态学特征 过氧化物酶同工酶 过氧化氢酶同工酶 超氧化物歧化酶同工酶 |
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| 基金项目:国家自然科学基金项目（39970112;30470268） |
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| Effects of microwave radiation on the ecological characteristics and antioxidant isoenzyme of Sitobion avenae Fabricius |
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| Abstract: |
| 【Objective】The effects of microwave radiation on the ecological characteristics and antioxidant isoenzyme of wheat aphid,Sitobion avenae Fabricius were analyzed to provide theoretical basis for Sitobion avenae population breakout and integrated management,and to lay a foundation for further study on toxigenicity mechanism of microwave radiation and genetic evolution of aphid population.【Method】The aphid was fed wheat leaves under the microwave radiation of different densities(2,4 and 6 W for each power,10,20 and 30 s for each radiation period).Time special life table of F2 was made,the effects of microwave on the ecological parameters of aphid were analyzed and the influence of microwave on antioxidant isoenzyme was shown through polyacrylamide gel electrophoresis (PAGE).【Result】Compared with the control,the mean development time (T) was prolonged at low and medium intensity(2,4 W) while the net reproductive rate (R0) and the intrinsic rate of increase (Rm) were markedly increased.On the other hand,under high intensity(6 W),the development duration was significantly shortened while the reproductive rate and the innate capacity of increase were dramatically reduced.Different intensities of microwave treatment also had a significant effect on wheat aphids’ antioxidant enzyme system from superoxide dismutase (SOD),peroxidase (POD) and catalase (CAT).Specific bands of the POD enzymes produced;the band intensity of SOD and CAT changed.【Conclusion】The variation of ecological parameters indicated that the detrimental effects of the microwave radiation on aphids were inherited by the next generation and the higher microwave radiation intensity showed greater impact.Peroxidase was obviously activated by microwave;however,CAT and SOD were inhibited. |
| Key words: microwave radiation Sitobion avenae Fabricius ecological characteristic peroxidase isozyme catalase isozyme superoxide dismutade isozyme |
