| 摘要: |
| 【目的】对普通小麦(Triticum aestivum L.) 品种7182与华山新麦草(Psathyrostachys huashanica)杂交后代中选育的矮秆种质B62进行农艺性状考察和外源遗传物质检测。【方法】以普通小麦-华山新麦草矮秆种质B62为材料，在田间农艺性状考察的基础上，采用细胞学及基因组原位杂交（根尖与花粉母细胞中期Ⅰ）技术，检测B62中的华山新麦草遗传物质。【结果】根尖细胞学鉴定表明，B62染色体条数为44条；根尖细胞染色体原位杂交（GISH）表明，B62携带2条华山新麦草染色体；花粉母细胞减数分裂Ⅰ中期染色体GISH结果显示，2条外源染色体能够配对，说明2条异源染色体是华山新麦草的1对同源染色体。【结论】矮秆种质B62为普通小麦-华山新麦草二体异附加系，其农艺性状优于其小麦亲本7182。 |
| 关键词: 普通小麦 华山新麦草 矮秆 异附加系 GISH分析 |
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| 基金项目:西北农林科技大学科研专项（Z109021002）；西北农林科技大学唐仲英育种基金项目（A212020716）；陕西省重大科技攻关“13115”专项（2007ZDKG-01） |
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| Molecular cytology on a dwarf germplasm derived from Triticum aestivum×Psathyrostachys huashanica |
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| Abstract: |
| 【Objective】The study was to observe the agronomy and alien genetic chromatin of a dwarf germplasm B62 obtained by screening the offspring of hybridizing between Triticum aestivumL.cv.7182 and Psathyrostachys huashanica.【Method】 Cytology analysis,genomic in situ hybridization (GISH) analysis on root tip and PMC Ⅰ of B62 were used.【Result】44 chromosomes were observed after identificating root tip in cytology.With P.huashanica Ns genomic DNA as a probe,root tip genomic in situ hybridization (GISH) analysis identified two material carries P.huashanica chromosomes.Then it was concluded that B62 was a wheat P.huashanica disomic or double monosomic addition line;and the addition line for pollen mother cell mid meiosisⅠgenomic in situ hybridization (GISH).The results show that the two alien chromosomes can pair and arrange in equatorial plate.It shows that the two added chromosomes are a pair of homologous chromosomes in P.huashanica.【Conclusion】 B62 is a dwarf material of the wheat P.huashanica hybridization disomic addition line,which agronomic are traits better than the wheat parent 7182. |
| Key words: Triticum aestivum Psathyrostachys huashanica dwarf alien addition line GISH analysis |
