| 摘要: |
| 【目的】克隆太行黑山羊成纤维细胞生长因子5（FGF5）基因CDs区，并对其进行序列特征分析和实时荧光定量表达检测。【方法】于2009年的7～10月份采集太行黑山羊的皮肤组织样品，利用RT-PCR方法克隆FGF5基因CDs区序列，并分析其序列特征及在不同生长发育时期的表达情况。【结果】太行黑山羊FGF5基因CDs区全序列长度为813 bp，编码270个氨基酸，其中包含一个FGF结构域（87～221位氨基酸）；太行黑山羊FGF5基因CDs区核苷酸序列与牛、马、人、猩猩和小鼠的同源性分别为98%，92%，90%，90%和86%，其编码氨基酸序列与牛、马、人、猩猩和小鼠的同源性分别为98%，91%，91%，91%和91%；实时荧光定量分析结果表明，在检测的4个月份的皮肤组织中，FGF5基因在9月份表达量显著高于7、8、10月份（P＜0.05），而7、8、10月份之间表达量无显著差异（P＞0.05）。【结论】太行黑山羊的FGF5基因编码的氨基酸高度保守；太行黑山羊毛囊发育在7、8月份处于生长期，9月份由生长期向退行期过渡，10月份进入退行期。 |
| 关键词: 太行黑山羊 FGF5基因 RT-PCR 基因序列 氨基酸序列 |
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| 基金项目:农业部转基因生物新品种培育科技重大专项“高产绒量转基因绒山羊新品种培育”（2008ZX08008-002） |
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| Study on sequence characterization and expression pattern of Taihang black goat FGF5 CDs region |
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| Abstract: |
| 【Objective】 The CDs region of Taihang black goat Fibroblast Growth Factor5 (FGF5) gene was cloned and the sequence characterization and quantitative analysis were performed.【Method】 The skin tissue of Taihang black goat was collected in July,August,September and October.The CDs region was cloned from total RNA by RT-PCR.The sequence characterization and expression of follicle growth in different development periods of FGF5 gene were performed.【Result】 The CDs region of FGF5 in Taihang black goat consisted of 813 nucleotides encoding 270 amino acids with a FGF domain from 87-221 amino acids.The nucleotides sequence homology of FGF5 CDs of Taihang black goat was found to be 98%，92%，90%，90% and 86% compared with that of bovine,horse,human,orangutanand mouse,while the amino acid sequence homology was 98%，91%，91%，91% and 91% respectively；The result of real-time quantitative PCR showed:the FGF5 was highly expressed in September,significantly higher than July,August and October(P<0.05),but there was no significant difference between these three months (P>0.05).【Conclusion】 The amino acids coded by the gene FGF5 are highly conserved in Taihang black goat.The result of real time quantitative indicated that the follicle development of Taihang black goat is in anagen in July and August,followed by the transition of anagen and catagen in September,and catagen in October. |
| Key words: Taihang black goat FGF5 gene RT-PCR gene sequence amino acid sequence |
