| 摘要: |
| 【目的】研究中华蟾蜍变态过程中肠、肝和胰腺的形态及其显微结构的变化。【方法】采用解剖学和组织学方法，取变态前后5期(27，35，38，42期和46期)的中华蟾蜍蝌蚪各10只，解剖后观察，并测量体质量、消化管质量等数据。再分别取38和42期蝌蚪数只，制作石蜡组织切片观察组织结构的变化，利用Spss13.0分析所得数据。【结果】在38期，中华蟾蜍蝌蚪消化管最长，在38～42期消化管的长度平均缩短75%左右。体质量在38期前呈增加趋势，38～46期则迅速下降；肠比重（I/B）在38期最大；在整个试验期内，肝胰比重（LP/B）变化不显著；变态高峰后与变态高峰前相比，胃壁厚度显著减小，肝细胞密度显著增大，肠壁厚度变化不显著。【结论】中华蟾蜍消化系统组织结构的变化与变态发育过程中食性的变化相适应。 |
| 关键词: 中华蟾蜍 消化系统 变态发育 |
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| 基金项目:陕西省自然科学基金项目（SJ08C101）；中央高校基本科研业务一般项目（GK200902039） |
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| Digestive system remodeling during metamorphosis of Bufo gargarizans Tadpoles |
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| Abstract: |
| 【Objective】The morphology and microstructure changes of intestine,liver and pancreas of Bufo gargarizans in metamorphosis were researched.【Methed】Anatomical and histological methods were used in this study,10 tadpoles were taken from every five different stages before and after metamorphosis（stage27,stage35,stage38,stage42 and stage 46）,and body weight,digestive tract weight and other data were measured after dissection,then several tadpoles at stage38 and stage 42 were selected for paraffin tissue section,and the changes in organizational structure were observed,the data were analysed by spss13.0.【Result】The results showed that the digestive tract was the longest at stage 38,and the length of digestive tract between the stage 38-stage 42 had an average reduction of about 75%.The body weight showed an upward trend before stage 38,while it dropped rapidly between stage 38-stage 46;and the maximum of intestinal weight compare with body weight(I/B) appeared at stage 38.Throughout the trial period,the total weight of liver and pancreas compare with body weight(LP/B) did not change significantly;the gastric wall thickness decreased significantly before and after metamorphosis, but the intestine wall thickness did not change significantly;the liver cell density increased significantly.【Conclusion】The changes in organization and structure showed that the characteristics of the digestive system adapted to the changes in feeding habits during metamorphosis. |
| Key words: Bufo gargarizans digestive system metamorphosis |
