| 摘要: |
| 【目的】证实前期消减文库的研究结果，揭示紫外诱导条件下麦长管蚜的生态遗传机理。【方法】根据前人研究证明的细胞色素b基因和SOD基因的保守性区域,设计特异性引物,采用RT-PCR方法，扩增紫外线照射24，48 h和对照麦长管蚜变异基因的部分序列，进行测序及核苷酸分析，并推导氨基酸序列。【结果】RT-PCR扩增分别得到长度420 bp的细胞色素b基因cDNA片段和357 bp的超氧化物歧化酶（SOD）基因cDNA片段。序列分析表明，细胞色素b基因编码140个氨基酸残基，30 W紫外辐射48 h处理的麦长管蚜细胞色素b基因突变位点为3个，其编码的氨基酸序列变异位点为2个；SOD基因编码119个氨基酸残基，且对照与处理的序列一致，未发现变异位点。【结论】紫外诱导条件下，麦长管蚜细胞色素b基因发生突变部分的具体位点已经找到，其突变方式为转换和颠换；SOD基因未发现变异位点。 |
| 关键词: 麦长管蚜 紫外诱导 RT-PCR 细胞色素b 超氧化物歧化酶（SOD） |
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| 基金项目:国家自然科学基金项目(39970112,30470268);中德农业合作项目（2006/2007(04)） |
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| Cloning and sequence analysis of cytochrome b gene and SOD gene in Sitobium avenae under the ultraviolet induction |
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| Abstract: |
| 【Objective】The purpose of this research is to confirm the results of early study and reveal the theory of Sitobium avenae’s ecological geneties under the ultraviolet induction for 24 and 48 h.【Method】According to the conserved regions of other known insects,a pair of specific primers were designed and the RT-PCR method was used to amplify the partial sequences of cytochrome b gene and SOD gene from S.avenae.The sequences were analyzed and the amino acid residues derived.【Result】Sequencing results showed that the cDNA fragment of cytochrome b gene was 420 bp in size and it encoded 140 amino acid residues,and the cDNA fragment of superoxide dismutas gene was 357 bp in size and it encoded 119 amino acid residues.The results displayed that the nucleotide sequence of cytochrome b gene in ones which were under ultraviolet induction 30 W 48 h had three mutant sites different from contrast ones,and made 2 mutant sites on acid residues.And the nucleotide sequence of superoxide dismutas gene were the same between UV induction ones and contrast ones.【Conclusion】Some specific mutant sites on cytochrome b gene of S.avenae under ultraviolet induction have been found.The mode of mutation is gene conversion or transversion.The SOD gene has no mutant sites. |
| Key words: Sitobium avenae ultraviolet induction RT-PCR cytochrome b superoxide dismutas |
