| 摘要: |
| 【目的】改良非致病性尖镰孢FO47的综合性状，获得具有较好防效的生防菌株。【方法】利用原生质体融合技术，将非致病性尖镰孢FO47和生防放线菌153进行融合，通过形态特征和菌落直径生长情况对融合菌株进行初筛，并采用西瓜苗期生长发育、防病效果和抗药性试验对所获得的初筛菌株进行复筛。【结果】筛选出14株菌落直径较FO47生长快的再生菌株，其中菌株F1-35、F1-38、F1-1和F1-41能够促进幼苗生长，菌株F1-35处理西瓜幼苗后单株鲜质量较FO47提高了36.93%，根长较FO47增加了19.07%。菌株F1-35、F1-38和F1-4对西瓜枯萎病的防治效果超过50%，其中菌株F1-35的防治效果最好，达到59.04%。菌株F1-4对多菌灵表现出较高的抗性，说明菌株F1-4可以与低质量浓度多菌灵混合使用防治西瓜枯萎病。【结论】筛选出3株综合性状得到提高的再生菌株，说明原生质体融合是改良生防菌株的有效手段。 |
| 关键词: 非致病性尖镰孢FO47 生防放线菌153 原生质体融合 |
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| 基金项目:教育部创新团队发展计划项目（200558）；国家高等学校学科创新引智计划项目（B07049）；西北农林科技大学创新团队项目 |
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| The improvement of Fusarium oxysporum FO47 by protoplast fusion |
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| Abstract: |
| 【Objective】The study was to improve the biocontrol character of non-pathogenic Fusarium oxysporum FO47.【Method】Protoplast fusion was carried out between FO47 and biocontrol actinomycetes 153.The recombinants were selected based on morphological and cultural character,colony diameter,greenhouse pot experiment and resistance to carbendazim.【Result】Fourteen recombinants were selected because the growth rates of colonies were faster than that of FO47. Strains F1-35，F1-38，F1-1 and F1-41 could promote the growth of the seedlings of watermelon.The fresh weight of seedlings of watermelon treated by strain F1-35 was more than 36.93%,and the lengh of root was more than 19.07% compared to strain FO47.The control effects of strain F1-35，F1-38 and F1-4 were more than 50%.The control effect of strain F1-35 was 59.04% and was the best.Strain F1-4 showed more resistance to carbendazim.The integration of strain F1-4 and low concentration of carbendazim was used to control F.oxysporum f.sp.niveum.【Conclusion】Three strains with excellent characers were selected indicating.Protoplast fusion is an effective tool to improve wild strains and the foundation of biocontrol of plant diseases. |
| Key words: Fusarium oxysporum FO47 biocontrol actinomycetes 153 protoplast fusion |
