| 摘要: |
| 【目的】对金黄节杆菌DR-536（Arthrobacter aurescens strain DR-536）分泌产生新型溶栓酶FA-Ⅰ的发酵条件进行优化。【方法】通过纤维蛋白平板法，测定不同氮源、碳源、复配氮源、金属离子、NaCl、初始pH、接种量、装液量、发酵时间和培养温度条件下菌株DR-536分泌的溶栓酶产量。【结果】菌株DR-536（A.aurescens strain DR-536）分泌产生溶栓酶FA-Ⅰ的最佳发酵培养基配方为：黄豆粉1.0%，酵母膏1.0%，NaCl 2.0%，FeSO4 0.05%，pH 6.0。最佳发酵条件为：接种量4%，装液量20 mL/150 mL，发酵时间72 h，发酵温度30 ℃。【结论】金黄节杆菌DR-536（A.aurescens strain DR-536）在普通培养条件下能够大量分泌溶栓酶FA-Ⅰ，适于大规模发酵，便于该酶的开发利用。 |
| 关键词: 金黄节杆菌 溶栓酶 发酵条件 优化 |
| DOI： |
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| 基金项目:陕西省科技攻关项目(2006K02-G14-03) |
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| Optimization on fermentation of Arthrobacter aurescens strain DR-536 to secrete a novel fibrinolytic enzyme FA-Ⅰ |
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| Abstract: |
| 【Objective】The fermentation conditions of Arthrobacter aurescens strain DR-536 to secrete a novel fibrinolytic enzyme FA-Ⅰ were optimized.【Method】Through the fibrin plate method,the productivity of fibrinolytic enzyme FA-Ⅰsecreted by strain DR-536 under different nitrogen sources,carbon sources,combined nitrogen sources,metal ions,NaCl,initial pH,inoculation volume,medium volume,fermentation time and temperature was measured.【Result】The optimal components of medium for strain DR-536 secreting FA-Ⅰwere as follows:soybean powder 1.0%,yeast extract 1.0%,NaCl 2.0%,FeSO4 0.05% and pH 6.0.The optimal fermentation conditions were as follows:inoculation volume 4%,medium volume 20 mL/150 mL flask,incubation time 72 h and temperature 30 ℃.【Conclusion】A.aurescens strain DR 536 could produce a large number of fibrinolytic enzyme FA-Ⅰ under normal fermentation conditions,making it convenient to utilize FA-Ⅰ as a thrombolysis agent. |
| Key words: Arthrobacter aurescens fibrinolytic enzyme fermentation optimization |
