| 摘要: |
| 【目的】研究急性持续性热辐射对小鼠皮肤热应激相关基因表达的影响，探讨热损伤的分子机制。【方法】将雄性Balb/c小鼠置于烤箱内，分别设定环境温度为43～61 ℃(以2 ℃递增)，进行急性持续性热辐射损伤，直至小鼠死亡，以室温下放置5 min的小鼠为对照组（CK），立即解剖致死小鼠，将去除毛发后的小鼠皮肤于体积分数为0.1% DEPC水中快速漂洗后，置液氮中保存。提取RNA并反转录，用两重RT-PCR扩增与热应激相关的基因并进行图像分析。【结果】hsp70、krt6α和thbs1等基因的表达，在不同温度热辐射作用下均较CK呈明显上调趋势。其中hsp70的表达在环境温度低于55 ℃时急剧上调，而在高于57 ℃时表达上调相对趋缓；而krt6α和thbs1的表达上调程度则与温度变化关系不大。此外，hsp105的表达与CK相比，在较低温度(51 ℃以下)时呈明显上调趋势，而在较高温度(53 ℃以上)时无明显差异。【结论】不同强度急性持续性热辐射对小鼠皮肤热应激相关基因的表达具有显著影响，且不同基因的表达量与温度之间呈现不同的相关性。同时，根据两重RT-PCR分析结果可知，hsp70可作为量化不同强度热辐射损伤的分子标记。 |
| 关键词: 热辐射 RT-PCR hsp70 小鼠 |
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| 基金项目:国家科技重大专项课题；军事医学科学院科技创新基金；毫米波国家重点实验室开放课题(K200807) |
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| Effects of acute and continuous heat exposure on expression of heat shock related genes in mice skin |
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| Abstract: |
| 【Objective】The study was done to investigate the effects of acute and continuous heat exposure on expression of heat-shock related genes in mice skin in order to explore the molecular mechanism of heat injury.【Method】Animals were placed in an oven with different temperatures preestablished (43-61 ℃ with an interval incremental of 2 ℃) to receive acute and continuous heat exposure until death,and the control was in the room temperature condition.Immediately after dissecting the dead mice,the mice skin was quickly rinsed with 0.1% DEPC water and saved in liquid nitrogen before use.RT-PCR was then used to examine the heat shock related genes expression in mice skin followed with image analysis.【Result】Results showed that expression of hsp70,krt6α and thbs1 was significantly upregulated in the temperatures examined compared with the control.The upregulation of hsp70 affected by the temperatures for exposure showed a dynamic pattern but not for krt6α and thbs1.In short,the upregulation of hsp70 was extremely rapid when the temperatues were below 55 ℃ but slow when above 57 ℃.In addition,compared with the control,the upregulation of hsp105 could be observed in relatively lower temperatures (51 ℃ and below) but not in higher temperatures (53 ℃ and above).Therfore,there were also certain relations with acute high heat and acute low heat.【Conclusion】In conclusion,our data suggested that acute and continuous heat exposure significantly influenced the expression of heat shock related genes in mice skin,while the expression patterns of different genes were largely related with the temperatures during heat exposure.Additionally,hsp70 might be considered as a biomarker to quantify the degrees of heat exposure injury in the body. |
| Key words: heat irradiation RT-PCR hsp70 mice |
