| 摘要: |
| 【目的】对根癌土壤杆菌(Agrobacterium tumefaciens)DK-24发酵生产辅酶Q10的培养基进行优化，为辅酶Q10的生产提供技术支持。【方法】用Plackett-Burman设计法(Plackett-Burman design,P-B)筛选对辅酶Q10产量影响较大的主要因子，采用最陡爬坡试验逼近主要因子的最优水平，通过Box Behnken设计，利用Design-Expert软件进行回归分析，优化确定各主要因子的最佳质量浓度。【结果】根癌土壤杆菌生产辅酶Q10的4个主要影响因子为蔗糖、玉米浆干粉（CSP）、酵母膏和对羟基苯甲酸（PHB）；最陡爬坡试验、Box-Behnken设计及响应面分析表明，根癌土壤杆菌DK-24发酵生产辅酶Q10的发酵培养基中，蔗糖、CSP、酵母膏和PHB的最优质量浓度分别为46.56，22.30，13.46 g/L和52.38 mg/L。【结论】在优化培养基条件下，辅酶Q10产量可达24.97 mg/L，较优化前提高了76.18%。 |
| 关键词: 根癌土壤杆菌 辅酶Q10 发酵培养基 发酵条件优化 |
| DOI： |
| 分类号: |
| 基金项目:国家“十一五”科技支撑计划项目（2006BAK02A18,2006BAK02A48,2006BAK02A05）；农业部跨越计划项目（2005-4）；陕西省重大科技专项 （2006KZ09-G1,2008ZDKG-04） |
|
| Optimization of nutrient composition for enhanced production of coenzyme Q10 using Agrobacterium tumefaciens DK-24 |
|
|
| Abstract: |
| 【Objective】This research optimized the culture components for coenzyme Q10 production by Agrobacterium tumefaciens DK-24,in order to provide technical support for industrial scale production of coenzyme Q10.【Method】In the first step,Plackett-Burman Design was used to evaluate the influence of twenty related factors.The path of steepest ascent was used to approach the optimal region of the fermentation conditions subsequently.In the third step,the concentrations of those main factors were further optimized by using Box Behnken Design and Response Surface Analysis.【Result】Sucrose,corn steep powder (CSP),yeast extract and PHB with great influence on coenzyme Q10 fermentation were chosen.By solving the quadratic regression model equation,the optimal concentrations of the variables were determined.The optimal medium for coenzyme Q10 production consisted sucrose,46.56 g/L;CSP,22.30 g/L;yeast extract,13.46 g/L;PHB,52.38 mg/L.【Conclusion】Under the optimal culture conditions,the coenzyme Q10 yield reached 24.97 mg/L,which was 76.18% higher than that achieved in the original medium. |
| Key words: Agrobacterium tumefaciens coenzyme Q10 nutrient composition optimization of fermertation condition |
