| 摘要: |
| 【目的】克隆小麦蓝矮病（WBD）植原体β-1,4-内切葡聚糖酶基因（FrvX），验证其在植原体侵染寄主中的作用，为更好地防控小麦蓝矮病奠定理论基础。【方法】采用PCR技术从小麦WBD植原体中扩增到FrvX全基因，将其插入到病毒载体pgR107中，构建重组病毒载体pgR107-FrvX，用农杆菌介导法侵染本氏烟草，观察烟草表型的变化。【结果】FrvX基因全长1 071 bp，编码356个氨基酸。重组病毒载体接种烟草22 d后，有9株烟草植株表现出明显的花叶、黄化症状，RT-PCR检测到发病植株接种7 d后的叶片有FrvX基因的转录。【结论】FrvX基因接种烟草导致烟草表型发生变化，推测其与植原体病害的发生有关。 |
| 关键词: 小麦蓝矮病植原体 β-1,4-内切葡聚糖酶基因 序列分析 致病性测定 |
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| 基金项目:国家自然科学基金项目（30871625）；公益性行业（农业）科研专项“稻麦重要病毒病株鉴定和防控技术体系研究”（nyhyzx07-051）；高等学校学科创新引智计划项目（B07049） |
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| Cloning and pathogenicity analysis of endo-1,4-beta-glucanase gene （FrvX）from wheat blue dwarf phytoplasma |
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| Abstract: |
| 【Objective】In order to effectively control the wheat blue dwarf(WBD) phytoplasma and identify the WBD phytoplasma has the similarities to known phytoplasma virulence factors,we cloned the endo-1,4-beta-glucanase (FrvX) gene from WBD.【Method】We amplified the endo-1,4-beta-glucanase (FrvX) gene from WBD by polymerase chain reaction,then inserted the FrvX gene to double expression virus vector pgR107 and inoculated tobacco through agrobacterium to observe symptoms manifested.【Result】The FrvX gene from WBD phytoplasma was 1 071 bp in length,encoding a predicted protein of 356 amino acids. Inoculated tobacco with pgR107 FrvX showed shrinking and mottle after 22 days,we detected FrvX gene were transcripted in infected plant after 6 days.【Conclusion】The phenotype of tobacco changed after FrvXgene were inoculated,so we infer it related to the occurrence of phytoplasma disease. |
| Key words: wheat blue dwarf phytoplasma endo-1,4-beta-glucanase sequencing analysis pathogenicity analysis |
