| 摘要: |
| 【目的】对山东省鸡鼻气管鸟杆菌进行分离鉴定。【方法】采集山东省不同地区有呼吸道症状的病（死）鸡，进行鼻气管鸟杆菌（Ornithobacterium rhinotracheale,ORT）的分离，根据培养特性及生化试验和玻片凝集试验结果对分离的菌株进行鉴定；应用PCR方法对分离菌株16S rRNA基因的一段保守序列进行扩增，应用琼脂扩散沉淀试验（AGP）对分离菌株的血清型进行鉴定，并对分离株进行药敏试验。【结果】 试验共分离到5株ORT，所有分离菌株均能扩增到1条长671 bp的特异性片段，而阴性对照菌株的扩增结果为阴性；5株ORT分离菌株可分为3个血清型，其中血清A型3株、血清B型1株、血清D型1株；分离ORT菌株对羟氨苄青霉素、头孢曲松、丁胺卡那、氧氟沙星、恩诺沙星、环丙沙星高度敏感。【结论】从山东省病死肉鸡中分离鉴定到5株ORT。 |
| 关键词: 鼻气管鸟杆菌 分离鉴定 山东省 鸡 |
| DOI： |
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| 基金项目:山东省自然科学基金项目（Y2006D10） |
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| Isolation and Identification of Ornithobacterium rhinotracheale |
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| Abstract: |
| 【Objective】Ornithobacterium rhinotracheale(ORT) from Shandong province was isolated.【Method】ORT,a causative agent of respiratory disease in fowl,was isolated from different commercial flocks showing respiratory symptoms in Shandong province.A slide agglutination test was used to detect ORT.PCR was performed to detect ORT.In order to determine the serotype of ORT,an agar gel precipitation test was performed and Antimicrobial susceptibility was also tested.【Result】Five strains were isolated.All of the isolates gave a positive reaction to the ORT antiserum.A 671 bp fragment of the 16S rRNA gene was amplified from all of the five isolates.Three serotypes were identified:three of the isolates were serotype A,one isolate-serotype B and one-serotype D.All of the isolates were found to be susceptible to Amoxicillin,Ceftriaxone,Amikacin,Ofloxacin,Enrofloxacin and Ciprofloxacin.【Conclusion】Five strains of ORT were isolated from Shandong province. |
| Key words: Ornithobacterium rhinotracheale(ORT) isolation and identification Shandong province chicken |
