| 摘要: |
| 变性梯度凝胶电泳（DGGE）技术目前已被广泛应用于环境微生物领域。介绍了DGGE的基本原理和技术步骤，分析了该方法的主要影响因素及其优点和存在的问题；概述了PCR-DGGE技术在环境微生物领域，包括在微生物处理技术、微生物生态多样性研究和功能菌种优化筛选中的应用现状，并展望了其应用前景。 |
| 关键词: 变性梯度凝胶电泳 微生物处理 微生物多样性 菌种筛选 |
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| 基金项目:2009年天津市自然科学基金重点项目（09JCZDJC26200）；天津市科委自然科学基金项目(043611111) |
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| PCR-DGGE and its application in the research of environmental microbiology |
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| Abstract: |
| The denaturing gradient gel electrophoresis (DGGE) has been proven to be widely used in the microbial environment,including the microbial treatment,microbial diversity and the screening of the function bacterium.In this paper,the principle and technical route of DGGE is briefly introduced,including its main causing factors,the excellence and the limitation.The future of this technology is also expected. |
| Key words: DGGE microbial treatment microbial diversity bacterium screening |
