| 摘要: |
| 【目的】通过表型分析筛选与苹果果实酸/低酸性状基因连锁的SSR分子标记，分析苹果果实可滴定酸的遗传规律，为苹果的早期选择和辅助育种提供依据。【方法】以“富士”ד粉红女士”杂交F1代的76株群体为试材，测定了成熟苹果果实的可滴定酸含量，采用BSA法和SSR技术筛选与酸/低酸性状基因连锁的分子标记，并在杂种后代中进行验证。【结果】76株杂交F1代群体可滴定酸含量为1.4～8.8 mg/g，从80对共显性遗传的SSR引物中筛选到与酸/低酸性状基因连锁的SSR标记Hi01e10，其遗传距离为10.014 cM，在杂交后代中的验证结果与可滴定酸含量在后代群体中的遗传规律基本吻合，进一步验证了该标记的可靠性。【结论】苹果果实酸/低酸性状是受主基因(Ma/ma)控制的，SSR标记Hi01e10可用于苹果的早期选择和辅助育种。 |
| 关键词: 苹果 酸/低酸性状 SSR标记 可滴定酸含 |
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| 基金项目:国家现代农业技术体系专项（nycytx-08-01-03） |
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| SSR makers of apple fruit acid/low-acid trait |
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| Abstract: |
| 【Objective】In order to provide evidence for early selection and molecular marker assisted selection of apple,the titratable acid genetic characteristics were researched by SSR marker analysis and titratable acid analysis.【Method】The apple hybrid combinations (“Fuji”דPinklady”) and 76 F1 generation groups were used to determine the mature fruit titratable acid content，the linked marker was screened by BSA method and SSR technology.【Rusult】A SSR marker Hi01e10 linked to acid/low-acid trait gene was screened from 80 SSR primer pairs by analyzing the genetic characteristics of 76 F1 generation groups,the genetic distance was 10.014 cM.The validity of this SSR maker was verified by F1 population hybrid tests and its genetic characteristics of titratable acid.【Conclusion】The acid/low-acid trait gene was governed by a major gene,and Hi01e10 can be used in early selection and molecular marker assisted selection. |
| Key words: apple fruit acid/low-acid trait SSR marker content of titratable acid |
