| 摘要: |
| 【目的】 研究自然条件下，虫害枣树挥发物对邻近枣树直接防御反应的激发作用。【方法】 以生长健壮、长势一致的枣树（Ziziphus jujuba Mill.）和3龄枣镰翅小卷蛾（Ancylis SativaLiu，又名枣粘虫）为试验材料，测试虫害枣树、邻近枣树及对照枣树叶片中蛋白酶抑制剂（Plant protease inhibitors，PI）、多酚氧化酶（Polyphenoloxidase，PPO）和脂氧合酶（Lipoxygenase，LOX）3种直接防御酶活性的变化。【结果】 枣树在受到枣镰翅小卷蛾幼虫危害24 h后，虫害株和邻近株的PI、PPO和LOX 3种酶活性均显著高于对照株，邻近株的PI、PPO和LOX活性分别较对照株高11.93%，19.91%和17.0%。枣镰翅小卷蛾幼虫危害3 d后，虫害株和邻近株3种酶活性均显著高于对照株，且邻近株PI活性呈曲折型下降，PPO活性呈“W”形变化，LOX活性则呈“N”形变化。从防御反应持续时间上看，邻近株PI高活性（与对照株相比）可持续5 d，PPO和LOX高活性可持续5 d以上；虫害株3种酶的高活性持续时间更长，强度更大。【结论】 虫害枣树挥发物激发了邻近枣树的直接防御反应，显著提高了邻近株的防御水平，防御状态持续时间在5 d以上。 |
| 关键词: 虫害挥发物 枣树 防御反应 激发作用 |
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| 基金项目:国家“十一五”科技支撑计划项目（2006BAD01A1701）；农业部公益性行业专项（200803006） |
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| Direct defense responses of healthy jujube primed by herbivore induced plant volatiles |
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| Abstract: |
| 【Objective】 Priming of direct defense responses in neighboring unattacked jujube induced by herbivore induced plant volatiles was determined in nature.【Method】 Taking Jujubes (Ziziphus jujuba Mill.) and jujube armyworms (Ancylis sativa Liu) as materials,we mainly detected activities of three direct defense enzymes，plant protease inhibitor (PI),polyphenol oxidase (PPO) and lipoxygenase (LOX) in armyworm attacked jujube and its nearby heathy plants.【Result】 The results demonstrated that the activities of three direct defense enzymes in both attacked and neighboring jujubes were significantly increased after 24 h infested by armyworms,and the activites of PI,PPO and LOX in induced plant were 11.93%,19.91% and 17.0% higher than control respectively.After infested for 3 days,the activities of the defense enzymes in both jujube trees also significantly increased in nature.And the changes of PI,PPO and LOX activity were wavelike over 5 days.The activity of PI lasted 5 days in induced jujube while those of PPO and LOX persisted more than 5 days in infested plants.【Conclusion】 Herbivore-induced plant volatiles primed direct defense responses in nearby jujube,remarkably increased plant defenses which persisted more than 5 days. |
| Key words: herbivore-induced plant volatile jujube defense response priming |
