| 摘要: |
| 【目的】 准确地定量奶牛舍环境中气载微生物（细菌和真菌）的含量,并评估其健康风险。【方法】 利用国际标准的AGI 30空气采样器，以无菌生理盐水为采样介质，收集奶牛舍空气样品，分别用培养计数法和4，6-联脒-2-苯基吲哚（DAPI）染色法，对奶牛舍环境中气载微生物含量进行计数，比较两种方法的差异，并对气载微生物潜在危害进行评估。【结果】 培养计数法测得奶牛舍内气载微生物含量为1.14×105~8.32×106CFU/m3，而DAPI染色法为9.3×105~5.93×107 CFU/m3，DAPI染色法所测气载微生物含量约是培养计数法的7～733倍。以染色法测得的奶牛舍内气载微生物含量计算，人与奶牛每min吸入的微生物量分别为1.58×105 CFU和3.29×106 CFU。【结论】 染色计数法更能客观地反映环境中的气载微生物含量，有助于对环境的健康风险进行科学评估。 |
| 关键词: 奶牛舍 气载微生物 培养计数法 DAPI染色法 |
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| 基金项目:2009年国家国际合作项目“动物疫源性人畜共患病的监测”（2009DFA32890） |
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| Concentration detection of airborne microbes in cow environments |
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| Abstract: |
| 【Objective】 The research was conducted to quantify the concentrations of airborne microbes(bacteria and fungi) in cow houses and assess health risks.【Method】 Air samples of cow environments were collected by international AGI 30 using sterile saline water as sampling media;the concentrations of airborne microbes were counted by culture counting method and 4,6-diamidino-2-phenylindole(DAPI) staining counting method,respectively.The differences between the two methods were compared.【Result】 According to counting grown colonies,the concentrations of cow houses were within a range of 1.14×105-8.32×106 CFU/m3.The direct counting concentrations were between 9.3×105-5.93×107 CFU/m3.The results of DAPI staining counting method were about 7-733 times higher than those of culture counting method.Based on the concentration of DAPI staining counting method,the airborne microbes inhaled by a person and a cow were 1.58×105 CFU/min and 3.29×106 CFU/min,respectively.【Conclusion】 DAPI staining counting method could reflect the accurate concentrations of airborne microbes in the environment,and assess health risks scientifically. |
| Key words: cow house airborne microbe culture counting method DAPI-staining method |
