| 摘要: |
| 【目的】对脱酚棉籽蛋白限制性酶解因素进行分析，为提高棉籽蛋白的溶解性提供参考。【方法】 采用胰蛋白酶对脱酚棉籽蛋白（CPF）进行限制性酶解，以蛋白质的溶解度和水解度为指标，研究浸泡时间、搅拌方式以及酶解因素（酶用量、体系pH、底物（CPF）质量浓度、酶解温度、时间）对棉籽蛋白水解的影响。【结果】 将棉籽蛋白于酶解前在3倍量（mL/g）去离子水中浸泡3 h有利于酶解。在酶用量为75 kU/g、pH为8、底物质量浓度为80 g/L、50 ℃条件下磁力搅拌（120 r/min）酶解3 h，可得到水解度为10.67%、溶解度为54.47%的酶解棉籽蛋白。溶解度分析结果表明，限制性酶解棉籽蛋白在pH 1~11时具有较好的溶解度，且保持了棉籽蛋白酸溶性的特点。【结论】 用胰蛋白酶限制性水解脱酚棉籽蛋白，既可以显著提高棉籽蛋白的溶解度，又保持了其酸溶性的特点。 |
| 关键词: 胰蛋白酶 限制性酶解 棉籽蛋白 水解度 溶解度 |
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| 基金项目:安徽高校省级自然科学研究项目（KJ2009B146）；安徽省应用化学省级重点学科建设项目（No.200802187C）；滁州学院博士科研启动基金（2007-12） |
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| Parameter analysis of limited enzymolysis to de gossypol cottonseed protein |
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| Abstract: |
| 【Objective】 To provide some references for improving the solubility of cottonseed protein, the limited enzymolysis parameters of de gossypol cottonseed protein were analyzed.【Method】 The cottonseed protein was partially hydrolyzed by trypsinase.Taking solubility and degree of hydrolysis (DH) as evaluation index,the steeping time before enzymolysis,mixing ways and the enzymolysis parameters (such as trypsinase concentration,substrate concentration,pH value and temperature) were investigated in this paper.【Result】 Steeping the cottonseed protein flour in three times de ionized water (mL/g) before enzyme hydrolysis is helpful to enzymolysis.In this test,a proper DH (10.67%) and a higher solubility (54.47%) of cottonseed protein can be obtained on the condition of trypsinase concentration 75 kU/g,pH value-8,CPF mass concentration 80 g/L,the temperature 50 ℃ and hydrolysed 3 h with magnetic force stiring (120 r/min).The results of solubilty analyzed in different pH solutions show that the enzymolytic cottonseed protein has higher solubility in pH value from 1 to 11,and it still maintains the acid soluble character of cottonseed protein before enzymolysis.【Conclusion】 The solubility of de gossypol cottonseed protein can be significantly improved by limited hydrolyzed with trypsinase in proper condition,and the acid soluble character of cottonseed protein is remained. |
| Key words: trypsinase limited enzymolysis cottonseed protein degree of hydrolysis solubility |
