| 摘要: |
| 【目的】探索一种利用甘蓝胞质雄性不育系（CMS）下胚轴和子叶进行快速扩繁的技术，为利用CMS大量繁制甘蓝杂交种提供一条新途径。【方法】 以甘蓝CMS 05-2-10为试材，将苗龄5～7 d无菌苗的下胚轴和子叶切下，接种于MS培养基上，45~50 d后统计再生芽数，比较6 BA与NAA不同质量浓度配比对不定芽的诱导情况；将诱导出的不定芽接种于添加不同质量浓度（0，0.1，0.3，0.5 mg/L）NAA的MS生根培养基上，20 d后比较生根率和根的长势。【结果】 在对下胚轴和子叶进行不定芽诱导时，MS培养基中分别添加1.0和4.0 mg/L的6 BA对下胚轴、子叶的诱导效果较好，其芽再生频率和分化系数分别为83.3%，53.0%和3.6，3.0。在MS培养基中添加1.0 mg/L 6-BA+0.2 mg/L NAA，其对下胚轴不定芽的诱导效果较好，芽再生频率为80.7%，分化系数为3.4，褐化率为1.5%；在MS培养基中添加4.0 mg/L 6-BA+0.3 mg/L NAA，其对子叶不定芽的诱导效果较好，芽再生频率为50.0%，分化系数为3.2，褐化率为3.4%；二者的褐化率均明显低于仅添加6 BA的处理。在MS生根培养基中添加0.3 mg/L NAA时，不定芽的生根效果较好，生根率达100%，且根系生长健壮。【结论】 利用甘蓝胞质雄性不育系CMS 05-2-10的下胚轴和子叶作为外植体进行不定芽的诱导，对于下胚轴，其适宜培养基为MS+1.0 mg/L 6-BA+30 g/L蔗糖+8 g/L 琼脂；对于子叶，其适宜培养基为MS+4.0 mg/L 6-BA+30 g/L蔗糖+8 g/L 琼脂。不定芽诱导生根的最适培养基为MS+0.3 mg/L NAA+30 g/L蔗糖+8 g/L琼脂。 |
| 关键词: 甘蓝 胞质雄性不育系 下胚轴 子叶 离体培养 植株再生 |
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| 基金项目:国家“十一五”科技支撑计划项目（2008BADB1B02,2006BAD01A7-2-04）；陕西省“13115”重大科技专项（2008ZDKG-03）；农业部公益性行业（农业）科研专项经费项目（nyhyzx07-007） |
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| Study on tissue culture and plant regeneration of hypocotyl and cotyledon of cytoplasmic male sterile line in cabbage |
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| Abstract: |
| 【Objective】 This experimentation aimed to explore fast propagation technology using hypocotyl and cotyledon of cytoplasmic male sterile line in cabbage to offer a way to produce hybrid cabbage by using cytoplasmic male sterile line.【Method】 Cytoplasmic male sterile CMS 05-2-10 of cabbage was studied.Hypocotyl and cotyledon taken from 5-7 d seedling were inoculated in MS medium.The number of adventitious buds after 45-50 days was counted,and the result of hormone combinations of 6-BA and NAA on adventitious buds of induction was compared;adventitious buds were inoculated on MS medium supplement with different concentrations（0，0.1，0.3，0.5 mg/L）of NAA.The condition of rooting rate and growth vigour of root was analyzed.【Result】 When adventitious bud of hypocotyl and cotyledon were induced，the effects of adding 1.0 and 4.0 mg/L 6 BA in MS medium on adventitious bud regeneration were better,shoot regeneration frequency and coefficient of differentiation were 83.3%,53.0%and 3.6,3.0 respectively.The effect of adding 1.0 mg/L 6-BA+0.2 mg/L NAA in MS medium on adventitious bud regeneration of hypocotyl was better,shoot regeneration frequency was 80.7%,coefficient of differentiation 3.4,browning rate 1.5%;The effect of adding 4.0 mg/L 6-BA+0.3 mg/L NAA in MS medium on adventitious bud regeneration of cotyledon was better,shoot regeneration frequency was 50.0%,coefficient of differentiation 3.2,browning rate 3.4%.Both of them can significantly reduce browning rate in comparison with only adding 6 BA.The effect of adding 0.3 mg/L NAA in MS medium on rooting culture was better,rooting rate of adventitious bud reached 100%,and the root can grow strongly.【Conclusion】 Taking the hypocotyl and cotyledon of cytoplasmic male sterile CMS 05-2-10 of cabbage as explants to induce adventitious bud,the suitable medium on adventitious bud regeneration of hypocotyl is MS+1.0 mg/L6-BA+30 g/L sucrose+8 g/L agar;the suitable medium on adventitious bud regeneration of cotyledon is MS+4.0 mg/L 6-BA+30 g/L sucrose+8 g/L agar.The suitable medium for root induction is MS+0.3 mg/L NAA+30 g/L sucrose+8 g/L agar. |
| Key words: cabbage cytoplasmic male sterile line hypocotyl cotyledon tissue culture plant regeneration |
