| 摘要: |
| 【目的】 初步构建洋水仙的组培快繁体系，为洋水仙种球工厂化生产奠定基础。【方法】 以洋水仙品种Arkle为试验材料，选用其叶片、花茎及鳞茎的不同部位作为外殖体，以MS、1/2 MS和1/4 MS为基本培养基，分别添加不同质量浓度的a-萘乙酸（NAA）、吲哚丁酸（IBA）、2，4-二氯苯氧乙酸（2，4-D）、6-苄基腺嘌呤(6 BA)和活性炭（AC），研究不同外植体以及不同培养基对小鳞茎诱导、增殖及生根的影响。【结果】 以带鳞茎盘的双鳞片作外植体效果较好，鳞片部位以鳞茎外层鳞片的培养效果较好；较适宜的初代培养基为MS+6 BA 3.0 mg/L+NAA 0.5 mg/L+IBA 0.2 mg/L；较适宜的增殖培养基为MS+6 BA 1.5 mg/L+NAA 0.3 mg/L，其诱导率可达668.00%；适宜小鳞茎的生根培养基为1/2 MS+NAA 0.1 mg/L+1 g/L AC，其生根率可达到80%。【结论】 鳞片分化小鳞茎存在着部位效应；6 BA和NAA组合对小鳞茎的诱导和增殖有良好效果；1/2 MS与一定浓度的NAA和AC组合有利于生根。 |
| 关键词: 洋水仙 初代培养 增殖培养 生根培养 |
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| 基金项目:沈阳市科技攻关项目（1071146-3-00） |
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| Construction of rapid micropropagation system via in vitro culture for Narcissus cv.Arkle |
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| Abstract: |
| 【Objectives】 The aim of this study was to establish a Narcissus rapid tissue culture system initially and lay a certain foundation for factory production of Narcissus bulbs propagation.【Method】 We studied the effects of different explants and different media on bulblets induction,multiplication and rooting with Narcissus cv.Arkle as experimental material,its leaves,scapes and different parts of bulbs as explants,MS,1/2 MS and 1/4 MS,with different mass concentrations of the NAA,IBA,2,4 D,6 BA and AC as the basic media.【Result】 This study demonstrated that twin scale with basal plate was more suitable explant and the cultivation effect of external layer scale was better.The more appropriate medium for primary culture was MS+6 BA 3.0 mg/L+NAA 0.5 mg/L+IBA 0.2 mg/L.The proliferation medium was MS+6-BA 1.5 mg/L+NAA 0.3 mg/L,its induction rate being 668%.The rooting rate of the bulblets reached 80% on the medium of 1/2 MS+NAA 0.1 mg/L+1 g/L activated carbon.【Conclusions】 There was positive effect on scale differentiation to bulblets. The combination of 6-BA and NAA had favourable effects on induction and multiplication of bulblets.1/2 MS with a certain concentration of NAA and AC was beneficial to rooting. |
| Key words: Narcissus primary culture multiplication culture rooting culture |
