| 摘要: |
| 【目的】 筛选固始白鹅体尺性状的微卫星DNA标记。【方法】 测定固始白鹅的体尺性状。选用11对微卫星引物（WD136、WD206、G06、G07、CKW21、CKW23、TTUCG5、TTUCG4、TTUCG2、Aalu1、TTUCG1），对固始白鹅进行遗传检测，计算各微卫星位点的等位基因频率、多态信息含量、杂合度和有效等位基因数。利用最小二乘法拟合线性模型，探索11个微卫星位点与固始白鹅体尺性状的关系。【结果】 11个微卫星位点中的8个位点(WD136、WD206、G07、TTUCG4、CKW21、TTUCG2、TTUCG1和TTUCG5)在固始白鹅上有较好的多态性，其平均等位基因数为4.25（3～6）、平均杂合度为0.598（0.273～0.695）、平均多态信息含量为0.635（0.530～0.769）、平均有效等位基因数为3.345（2.383～4.984）。通过最小二乘分析, 检测到WD136、WD206、CKW21、TTUCG1等4个位点与9项体尺性状间存在显著相关性。【结论】 WD136位点有望作为体质量、体斜长、骨盆宽的早期选择辅助标记； WD206位点有望作为体质量、胸宽、龙骨长、骨盆宽、胫长、半潜水长和颈长的早期选择辅助标记；CKW21位点有望作为体质量、龙骨长、胫长、半潜水长、颈长的早期选择辅助标记；TTUCG1位点有望作为体质量、体斜长、胫长、龙骨长的早期选择辅助标记。 |
| 关键词: 微卫星标记 固始白鹅 体尺性状 |
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| 基金项目:国家蛋鸡产业技术体系项目；河南省杰出人才创新基金项目（074200510009） |
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| Screening of microsatellite DNA loci for body-size traits in Gushi goose |
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| Abstract: |
| 【Objective】 Microsatellite DNA loci for body-size traits in Gushi goose were screnned.【Method】 Genetic variations of eleven microsatellites (WD136,WD206,G06,G07,CKW21,CKW23,TTUCG5,TTUCG4,TTUCG2,Aalu1,TTUCG1) were detected in Gushi goose whose body size traits were determined.Allele frequency,heterozygosity,polymorphic information content,and the numbers of effective alleles of eleven microsatellite loci were calculated.The correlation between eleven microsatellite loci and body size traits in Gushi goose were preliminarily analyzed by least squares linear model.【Result】 The results indicated eight among eleven microsatellite loci were polymorphic with 4.25 alleles on average.The average heterozygosity was 0.598 and the average polymorphic information content was 0.635 and the average of effective alleles was 3.345.The results suggested there were significant links among WD136,WD206,CKW21,TTUCG1 microsatellite locus and nine body-size traits through least square estimate. 【Conclusion】 The result implied that marker WD136 might be regarded as candidate-locus for weight,body length and pelvis width of Gushi goose,marker WD206 might be regarded as candidate-locus for weight,chest width,sternum length,pelvis width,shank length,half-diving length and neck length of Gushi goose,marker CKW21 might be regarded as candidate-locus for weight,sternum length,half-diving length,shank length and neck length of Gushi goose,and marker TTUCG1 might be regarded as candidate locus for weight,body length,shank length and sternum length of Gushi goose. |
| Key words: microsatellite marker Gushi goose body-size trait |
