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| 单、双子叶作物间EST-SSRs引物和标记的通用性研究 |
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柴呈森1, 姜蔚1, 李娜1, 刘川1, 温雪梅1, 赵惠贤1, 胡胜武2
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1.西北农林科技大学 生命科学学院,陕西 杨凌 712100;2.西北农林科技大学 农学院,陕西 杨凌 712100
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| 摘要: |
| 【目的】 探讨单、双子叶作物间EST-SSR引物和标记的通用性。【方法】 选取30对小麦、8对油菜和14对白菜的EST-SSR引物，分别以10个小麦、10个油菜、5个大豆品种和5个玉米自交系的基因组DNA为模板，进行PCR扩增及扩增产物的多态性分析。【结果】 （1）30对小麦EST-SSR引物中，有29，28和26对引物分别在玉米、油菜和大豆中有扩增产物，可扩增率分别为96.7%，93.3%和86.7%；其中分别有12对和9对引物在单子叶作物小麦和玉米及双子叶作物大豆和油菜中的扩增产物显示多态性，有4对引物在4种作物中的扩增产物均显示多态性。（2）22对白菜/油菜EST-SSR引物中，有18，21和22对引物分别在大豆、小麦和玉米中有扩增产物，可扩增率分别为81.8%，95.4%和100%；其中分别有10对和7对引物在单子叶作物小麦和玉米及双子叶作物大豆和油菜中的扩增产物显示多态性，有7对引物在4种作物中的扩增产物均显示多态性。【结论】 在单、双子叶作物间开发可通用EST-SSR引物和建立可转化EST-SSR标记是可行的；在单、双子叶作物中均有多态性的EST-SSR标记所对应的基因，涉及贮藏蛋白、核酸的转录及复制、功能基因的表达调控、代谢催化等基础功能。 |
| 关键词: 单子叶作物 双子叶作物 EST-SSR 引物通用性 |
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| 基金项目:国家生命科学与技术人才培养基地科技创新基金项目(2007088);国家油菜产业化项目科学家岗位“化杀岗位”（nycytx-00505）;国家自然科学基金项目(30871578) |
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| The transferability of EST-SSR primers and markers between monocotyledonous and dicotyledonous crops |
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CHAI Cheng-sen,JIANG Wei,LI Na,LIU Chuan,WEN Xue-mei,ZHAO Hui-xian and HU Sheng-wu,CHAI Cheng-sen,JIANG Wei,LI Na,LIU Chuan,WEN Xue-mei,ZHAO Hui-xian and HU Sheng-wu,CHAI Cheng-sen,JIANG Wei,LI Na,LIU Chuan,WEN Xue-mei,ZHAO Hui-xian and HU Sheng-wu,CHAI Cheng-sen,JIANG Wei,LI Na,LIU Chuan,WEN Xue-mei,ZHAO Hui-xian and HU Sheng-wu,CHAI Cheng-sen,JIANG Wei,LI Na,LIU Chuan,WEN Xue-mei,ZHAO Hui-xian and HU Sheng-wu,CHAI Cheng-sen,JIANG Wei,LI Na,LIU Chuan,WEN Xue-mei,ZHAO Hui-xian and HU Sheng-wu,CHAI Cheng-sen,JIANG Wei,LI Na,LIU Chuan,WEN Xue-mei,ZHAO Hui-xian and HU Sheng-wu
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| Abstract: |
| 【Objective】 This study investigated the transferability of EST-SSR primers and markers between monocotyledonous and dicotyledonous crops. 【Method】 In the present investigation,30 pairs of EST-SSR primers from wheat,8 from oilseed rape and 14 from Chinese cabbage were selected according to the results reported previously，to conduct PCR with the genomic DNA from 10 wheat varieties,10 oilseed rape,5 soybean and 5 maize inbred lines as template,and to analyze the polymorphism of the PCR products.【Result】 (1)29,28,26 of 30 pairs of EST-SSR primers from wheat respectively had amplification products in maize,oilseed rape and soybean,the rate of amplification was 96.7%,93.3%,86.7%,respectively;12 of 30 pairs EST-SSR primers from wheat showed polymorphism in monocotyledons wheat and maize,9 in dicotyledons oilseed rape and soybean,and 4 in both monocotyledonous and dicotyledonous crops.(2)18,21,22 of 22 pairs EST-SSR primers from Chinese cabbage/rape respectively had amplification products in soybean,wheat and maize,the rate of amplification was 81.8%，95.4%，100%,respectively;10 of 22 pairs EST-SSR primers showed polymorphism in monocotyledons wheat and maize,13 in dicotyledons oilseed rape and soybean,7 in both monocotyledonous and dicotyledonous crops.【Conclusion】 These data indicate that it is feasible to establish universal primers and transferable EST-SSR markers between monocotyledonous and dicotyledonous crops.And it is found that these genes containing polymorphic markers based on EST-SSR in both monocotyledonous and dicotyledonous crops are associated with some basic functions,such as storage,nucleic acid transcription and duplication,regulation of the expression of the function genes,metabolize catalysis,and etc. |
| Key words: monocotyledonous crop dicotyledonous crop EST-SSR transferability of primer |
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