| 摘要: |
| 【目的】 探索小鼠子宫内膜细胞培养上清液对小鼠脾脏淋巴细胞和山羊外周血单个核细胞（Peripheral Blood Monouclear Cells,PBMC）的转化及分泌活性的影响。【方法】 采用噻唑蓝（MTT）比色法和酶联免疫吸附测定法（ELISA），分别研究小鼠子宫内膜上皮细胞（Endometrial Epithelial Cells,EECs）和基质细胞（Endometrial Stromal Cells,ESCs）培养上清液，对离体培养的小鼠脾脏淋巴细胞、山羊外周血单个核细胞（Peripheral Blood Monouclear Cells,PBMC）的增殖转化及白细胞介素2 (Interleukin-2,IL-2) 和白细胞介素4 (Interleukin-4,IL-4)分泌活性的影响。【结果】 小鼠子宫内膜基质细胞和上皮细胞培养上清液，均可无种属差异地刺激小鼠脾脏淋巴细胞和山羊PBMC的增殖转化，同时抑制植物血球凝素（Plant hemagglutimin PHA-P）诱导的小鼠淋巴细胞和山羊PBMC分泌IL-2的活性，但对PHA-P诱导的IL-4分泌有促进或协同作用。【结论】 EECs和ESCs通过影响小鼠脾脏淋巴细胞和山羊PBMC的增殖转化与分泌活性，优势活化Th2型淋巴细胞，在子宫局部免疫调节中发挥重要作用。 |
| 关键词: 子宫内膜细胞 淋巴细胞 外周血单个核细胞（PBMC） 白细胞介素-2 白细胞介素-4 小鼠 山羊 |
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| 基金项目:陕西省科技发展项目（2008K02-01）;西北农林科技大学博士点基金项目（200807120022） |
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| Effects of supernatant of mouse endometrial cells on proliferation and secretion activity of mouse spleen lymphocyte and goat PBMC |
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ZHOU Li-mei,HE Ya-mei,NIU Ya-jing,FU Sheng-yong,JIN Ya-ping
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| Abstract: |
| 【Objective】 The study was done to explore the effects of endometrial cells on proliferation and secretion activity of Mouse Spleen Lymphocyte and Goat PBMC the way of paracrine.【Method】 We studied the effects of the mouse endometrial epithelial cells (EECs),stromal cells (ESCs) culture supernatant on proliferation and secretion activities of IL-2 and IL-4 in vitro cultured mouse spleen lymphocyte,goat PBMC by MTT colorimetric test and ELISA method.【Result】 The supernatant from both the EECs and the ESCs could stimulate the proliferation of mouse spleen lymphocytes and goats PBMC and induce the secretion of IL-2 and IL-4,while inhibit the proliferation and IL-2 secretion of mouse spleen lymphocytes and goats PBMC induced by PHA-P,but enhance IL-4 secretion induced by PHA-P.【Conclusion】 The results revealed that EECs and ESCs affect the activation and secretion of mouse spleen lymphocytes and goat PBMC,predominantly the Th2 lymphocytes,in a paracrine way,thus play an important role in local uterine immune regulation. |
| Key words: uterus endometrial cell lymphocyte Peripheral Blood Monouclear Cell(PBMC) Interleukin-2 Interleukin-4 mouse goat |
