| 摘要: |
| 【目的】 从DNA分子水平研究‘早酥梨’及其极早熟芽变品种‘六月酥’的遗传差异，为极早熟梨的分子育种提供理论依据。【方法】 以‘早酥梨’和‘六月酥’梨的幼叶为试材，采用改良CTAB 法提取梨叶片基因组DNA，应用AFLP技术对‘早酥梨’和‘六月酥’进行遗传差异分析。【结果】 DNA完整性较好，纯度高。64对AFLP引物组合中有47对检测出DNA分子多态性，产生了1 931条清晰谱带，其中多态性位点有95个，多态性条带比例为9.38%，遗传相似系数为95.24%，差异片段主要集中在200～900 bp；47对不同引物组合均能在‘早酥梨’和‘六月酥’梨间检测到AFLP扩增差异位点。【结论】 ‘六月酥’和‘早酥梨’之间的差异与遗传物质的改变相关，其中一些差异可能与决定梨极早熟性状的基因有关。 |
| 关键词: 早酥梨 六月酥梨 芽变 AFLP |
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| 基金项目:陕西省“13115”科技创新工程项目（2008ZDKG-08）；陕西省科技攻关项目（2008K01-11）；西北农林科技大学科研启动项目 |
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| AFLP analysis of extra early mutant cultivar of pear ‘Liuyuesu’ |
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CHEN Zi xuana LI Zhan bina LI Penga YU Guo qiangb ZHU Bing bingb
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| Abstract: |
| 【Objective】 The genetic diversity was researched for pear cultivars ‘Zaosu’ and extra early mutant cultivar ‘Liuyuesu’ on molecular level,which would provide academic basis for the breeding of extra early pear on molecular level.【Method】 With young leaves of pear cultivars ‘Zaosu’ and ‘Liuyuesu’ as materials,improved CTAB method was used to extract high quality genomic DNA,and the AFLP marker technology was used to analyze the genetic diversity of pear cultivars‘Zaosu’ and ‘Liuyuesu’.【Result】 47 of 64 selective primer pairs amplified 1 931 clear bands,95 of them were polymorphic loci.The polymorphic rate and Neis similarity coefficient were 9.38% and 95.24% respectively.The range of the polymerphic frag ments was mainly 200-900 bp.【Conclusion】 The results indicated that there were some differentiation on molecular level between ‘Liuyuesu’ and ‘Zaosu’ pear.Some variations may be related with the genes deciding the early mature traits. |
| Key words: Zaosu pear Liuyuesu pear bud mutation AFLP |
