| 摘要: |
| 【目的】 研究番茄红素β-环化酶（LYC-b）基因反义表达对番茄果实中番茄红素含量的影响，为番茄品质育种提供新方法。【方法】 以番茄品种“TTI1117A”和“灵光3号”为材料，构建了分别由CaMV35S启动子驱动的GUS标记基因和反义LYC-b5′基因双价植物表达载体，通过农杆菌GV3101介导，将基因整合到番茄基因组中，采用GUS、PCR以及RT-PCR、Northern 杂交进行检测，并测定了转基因植株果实中的番茄红素含量。【结果】 由GUS、PCR检测结果可知，筛选出1株“TTI1117A”和4株“灵光3号”转基因植株；RT-PCR、Northern杂交检测结果表明，目的基因在RNA水平上已经表达；对番茄果实中番茄红素含量的测定结果表明，转基因植株果实中番茄红素含量均高于未转基因植株。【结论】 LYC-b基因的反义表达具有提高番茄果实中番茄红素含量的作用。 |
| 关键词: 番茄；番茄红素β-环化酶基因；反义表达 番茄红素含量 |
| DOI： |
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| 基金项目:陕西省“13115”科技创新工程重大科技专项（2007ZDKG-05，2009ZDKG-14）；西北农林科技大学育种专项（06YZ003） |
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| Effect of LYC-b gene antisense expression on lycopene content |
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WANG Fei quan XIAO Bin REN Hong lou LI Dong hua YAO Li juan WANG Zhen
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| Abstract: |
| 【Objective】 The study discussed the effect LYC-b gene antisense expression on lycopene content in order to provide new methods for the fruit quality breeding of tomato.【Method】 The two gene plant expression vectors including anti LYC-b5′ gene and GUS gene were constructed by using CaMV35S as a promoter and introduced into tomato cultivars TTI1117A and Lingguang No.3 by Agrobacterium tumefaciensGV3101 mediated transformation. 【Result】 GUS,and PCR detection showed that anti LYC-b5′ gene had integrated into genome of tomato.RT PCR,and Northern blot detection indicated that anti LYC-b5′ gene was expressed at RNA level.Lycopene content analysis of tomato fruit showed that lycopene content in transgenic fruit was higher than that of untransgenic fruit.【Conclusion】 LYC-bgene antisense expression can improve lycopene content of tomato fruit. |
| Key words: Lycopersicon esculentum Miller. LYC- gene antisense expression lycopene content |
