| 摘要: |
| 【目的】 研究p21WAF1/CIP1蛋白在成年小鼠卵泡发育与黄体形成及退化过程中表达的变化规律。【方法】 取20只成年雌性小鼠的卵巢，制作石蜡切片，经免疫组织化学SP法染色后，观察p21WAF1/CIP1蛋白在原始卵泡、初级卵泡、次级卵泡、三级卵泡、成熟卵泡、闭锁卵泡、早期黄体、中期黄体以及晚期黄体中的定位及表达。【结果】 卵泡发育和黄体形成及退化过程中均有p21WAF1/CIP1蛋白的表达；p21WAF1/CIP1蛋白在此过程中呈先升高后降低的趋势，峰值出现在三级卵泡时期。【结论】 p21WAF1/CIP1蛋白在卵母细胞中发挥一定作用，可能参与优势卵泡的选择，并对卵泡发育及黄体形成和退化有影响。 |
| 关键词: p21WAF1/CIP1 蛋白 卵泡发育 黄体形成与退化 |
| DOI： |
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| 基金项目:国家自然科学基金项目（30972151，30170683）；陕西省自然科学基金项目（2006C130） |
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| The expression of p21WAF1/CIP1 protein in adult mouse follicle and corpus luteum |
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ZHAO Shu juan，LEI Xue qin，XU Ting sheng，PANG You zhi，WANG Xiao xiao,ZHAO Shu juan，LEI Xue qin，XU Ting sheng，PANG You zhi，WANG Xiao xiao,ZHAO Shu juan，LEI Xue qin，XU Ting sheng，PANG You zhi，WANG Xiao xiao
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| Abstract: |
| 【Objective】 The study was done to investigate the changes of p21WAF1/CIP1 protein expression in adult mouse ovary during follicular development,corpus luteum formation and regression.【Method】 Ovarian tissues were obtained from 20 adult mice with normal estrus cycle.Paraffin slice and immunohistochemical SP method were applied for the expression and variation of p21WAF1/CIP1 protein in the periods of primordial,primary,secondary,tertiary,mature,atresic follicles,and forepart,metaphase and anaphase corpus luteums.【Result】 It was found that p21WAF1/CIP1 protein was expressd in all periods.The variation of p21WAF1/CIP1 protein in this process generally increased first and then decreased.The peak value appeared in the period of the tertiary follicle.【Conclusion】 p21WAF1/CIP1 protein plays a role in oocytes during follicular development,may participate in choosing dominant follicle,and has a potential influence on follicular development,corpus luteum formation and regression. |
| Key words: p21 WAF1/CIP1 protein follicular development corpus luteum formation and regression |
