| 摘要: |
| 【目的】 研究5-羟色胺（5-hydroxytryptamine，5-HT）在不同动情周期SD 大鼠子宫中的分布情况和变化规律，探讨5-HT在雌性生殖系统中的作用。【方法】 采用阴道涂片法鉴定动情周期，选取均为正常动情周期的20 只雌性SD大鼠，将其分为动情前期、动情期、动情后期和动情间期4组，处死各组大鼠，取子宫制作石蜡切片，经免疫组织化学超敏SP法染色后，观察 5-HT 在不同动情周期大鼠子宫中的分布及变化规律。【结果】 5-HT免疫阳性产物在子宫各层中均有不同程度的分布，其中主要存在于子宫内膜上皮细胞、子宫腺上皮细胞、血管内皮细胞、基质细胞以及子宫内膜固有层中的胚性结缔组织细胞中。子宫内膜固有层以椭圆形状和圆形的5-HT阳性细胞为主。此外，还有一些呈细丝状、串珠状的5-HT阳性神经纤维分布于子宫内膜固有层。环行肌层多为细丝状和串珠状且粗细不等的5-HT阳性神经纤维。纵行肌层5-HT阳性产物呈点状、细丝状、椭圆形状和串珠状分布。子宫内膜和肌层5-HT 阳性产物在动情期和动情间期均呈强阳性表达，而在动情前期和动情后期均呈弱阳性表达。【结论】 5-HT 在不同生理时期大鼠子宫中的表达分布存在一定规律性，可能与其生理调节功能有关。 |
| 关键词: 5-羟色胺 免疫组织化学SP法 动情周期 子宫 SD大鼠 |
| DOI： |
| 分类号: |
| 基金项目:陕西省自然科学基金项目(SJ08C105)；西北农林科技大学科研专项(06ZR003) |
|
| Distribution of 5-HT in uterus of rats during estrus cycle |
|
HAO Jian jun1 XU Yong ping1 ZHAO Hui ying1 PANG Guo liang2 GUO Cui xiang1 WANG Zhen1 LI Jun jin1 CHEN Yao1 NIU Gui qun1
|
| Abstract: |
| 【Objective】 Using Streptavidin Peroxidase（SP），the expression and variation of 5-hydroxytryptamine in uterus of the Sprague Dawley during estrus cycle were studied.【Method】 Twenty mature female SD rats of normal estrus cycle were randomly divided into 4 groups:preoestrus group,estrum group,metoestrus and diestrus group.Estrous cycle was judged by vaginal smear, the expression of 5-HT in uterus of the rats during estrus cycle was studied by immunohistochemical SP method.【Result】 The expression and variation of 5-HT in all parts of rat uterus varied and the immunoreactive products were mainly located in endometrial epithelial cells, adenomyos was epithelial cells,stromal cells,vascular endothelial cells,endometrial embryonic connective tissue cells.The majority part was elliptical and round 5-HT immunoreactive cells in endometrial tunicae propria.In addition，some filamentous and beaded 5-HT immunoreactive nerve fibers were distributed in the endometrial tunicae propria.In circular muscle layer，most of 5-HT immunoreactive products were filamentous and beaded thickness of 5-HT immunoreactive nerve fibers.In longitudinal muscle layer，5-HT immunoreactived products assumed punctate,filamentous, beaded and oval distribution.The 5-HT positive immunoreactive products of endometrium and mesometrium were strongly positive expressions in estrus and diestrus,but weaker in proestrus and metestrus.【Conclusion】 The results showed that the expression of 5-HT in the uterus in different periods was found of certain rules and might be related to its physiological function. |
| Key words: 5-HT immunohistochemical SP method estrus cycle uterus sprague-Dawley rat |
