| 摘要: |
| 【目的】 研制复方呋喃西林纳米乳，并检测其对奶牛子宫内膜炎的治疗效果。【方法】 用紫外分光光度计测定呋喃西林在3种油相（肉豆蔻酸异丙酯(IPM)、橄榄油、石蜡油）和2种表面活性剂（聚氧乙烯醚氢化蓖麻油（RH40）、Tween-80）中的溶解度，确定纳米乳处方。采用转相乳化法制备复方呋喃西林纳米乳，对其进行质量评价，并研究其对西安和北京2个奶牛场子宫内膜炎病牛的治疗效果。【结果】 复方呋喃西林纳米乳的配方是：呋喃西林 1%，替硝唑 0.3%，Tween-80 32.5%，Span-80 13.7%，橄榄油 5%，蒸馏水 47.5%（各组分含量均为质量分数）。用该配方制备的复方呋喃西林纳米乳为O/W型，乳滴呈球形，分布均匀，无粘连，平均粒径为67.20 nm，乳液澄清透明，避光保存时稳定性较好。复方呋喃西林纳米乳对西安某奶牛场子宫内膜炎病牛的治愈率为92.9%，较清宫液对照组高7.2%；有效率为92.9%，与清宫液治疗组相同。复方呋喃西林纳米乳对北京某奶牛场子宫内膜炎病牛的治愈率为66.7%，有效率为77.8%，均极显著高于宫速清溶液（P< 0.01）。【结论】 复方呋喃西林纳米乳研制成功，其对奶牛子宫内膜炎有较好的治疗效果。 |
| 关键词: 呋喃西林 复方纳米乳 奶牛子宫内膜炎 |
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| 基金项目:国家科技支撑计划项目（2006BAD04A11）；大学生创新性实验计划项目（071601004） |
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| Preparation of compound furacilin nanoemulsion and its clinic herapeutic effect on bovine endometritis |
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| Abstract: |
| 【Objective】 Compound furacilin nanoemulsion was prepared to invest its clinic therapeutic effect on the bovine endometritis.【Method】 The solubility of furanillin was measured respectively by UV spectrophotometer in three kinds of oils (IPM,olive oil,liquid paraffin ) and two kinds of surfactant (RH40,Tween-80),and then the optimal formulation was screened.Compound furacilin nanoemulsion was prepared by phase inversion emulsification and its quality was evaluated,and its clinic therapeutic effect on the bovine endometritis was studied.【Result】 Compound furacilin nanoemulsion could be prepared with furacilin 1%，tinidazole 0.3%，Tween-80 32.5%，Span-80 13.7%，olive oil 5%，and distilled water 47.5%（content of each constituent is quality fraction）.The nanoemulsion was the type of oil-in-water and transparent and uniform,nanoemulsion drop was spherical,well-distributed,no conglutinate with mean diameter 67.20 nm.Its stability was good when stored without light.Clinical test on cows with endometritis showed that the cure rate was 92.9%,7.2% higher than that of Qinggongye in control group,the effective rate being 92.9%,the same as Qinggongye in a dairy farm in Xi’an,and for that in Beijing the cure rate was 66.7% with the effective rate being 77.8%.The two values were significantly higher than that of GongSuqing in the control group respectively（P<0.05）.【Conclusion】 Compound furacilin nanoemulsion is prepared successfully,and the clinic therapeutic effect on the bovine endometritis is better. |
| Key words: furacilin compound nanoemulsion bovine endometritis |
