| 摘要: |
| [目的]研究逆境信号水杨酸(SA)刺激条件下,蚕豆气孔保卫细胞中H2O2的产生及其对气孔运动的影响.[方法]以蚕豆为材料,利用表皮条生物分析、膜片钳等方法研究SA诱导蚕豆气孔关闭过程中质膜内向K+电流的变化.[结果]SA可以浓度依赖的方式诱导蚕豆叶片的气孔关闭.质膜NADPH氧化酶抑制剂二亚苯基碘(DPI)可削弱SA作用的45%～60%.借助膜片钳手段记录SA处理条件下全细胞内向K+电流的变化,胞外0.1 mmol/L SA处理后20 min可抑制内向K+电流的70%左右.电极液中10μmol/L DPI作用20 min可逆转SA抑制全细胞内向K+电流的46%左右.[结论]SA诱导的气孔关闭可能与H2O2的产生有关,DPI逆转SA对保卫细胞内向K+电流的抑制作用暗示,H2O2可能通过抑制质膜内向K+电流而介导SA诱导的气孔关闭过程. |
| 关键词: 蚕豆；水杨酸；NADPH氧化酶；K+通道 膜片钳 |
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| 基金项目:河南省自然科学基金项目（0211031300） |
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| Effect of salicylic acid on stomatal movement and K+ current on plasma membrane of guard cell in Vicia faba L. |
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| Abstract: |
| 【Objective】 The effect of salicylic acid on stomatal movement and K+ channel on plasma membrane of guard cell in Vicia faba L.was studied.【Method】 Vicia faba L. as the material,epidermal strips bioassay and patch clamp were used to study Changes of K+ carrent on plasma meribrane in Vicia guard cells.【Result】 Salicylic acid (SA) can induce stomatal closure with a concentration-dependent manner.DPI 10 μmol/L weakened the effect of SA on stomatal apertures by 45%-60%,which indicated that the generation of H2O2 might be involved in the stomatal closure induced by SA.The voltage-dependent K+-selective channels in the plasma membrane were recorded by whole-cell configureuration by patch clamp.The whole-cell inward K+ currents were inhibited by 70% after 20 min treatment by SA 100 μmol/L.But intracellular application of DPI partly abolished SA-inhibited inward K+ current by 46% after 20 min treatment by SA.【Conclusion】 This indicated that H2O2 mediated SA-induced stomatal closuring by targeting inward K+ channels on plasma membrane. |
| Key words: Vicia faba L. salicylic acid NADPH oxidase K+ channel patch clamp |
