| 摘要: |
| [目的]克隆内蒙古绒山羊SRY基因的HMG-box区,并对其序列特征进行分析.[方法]参考GenBank中山羊SRY基因序列设计1对引物,采用PCR法扩增内蒙古绒山羊雄性个体的SRY基因HMG-box区全部序列,克隆到pMD18-T 载体中,转化DH5α菌,蓝白斑筛选阳性克隆,并进行测序.将测序结果与GenBank中部分偶蹄目动物SRY基因HMG-box的序列进行同源性比较,构建系统进化树.[结果]内蒙古绒山羊SRY基因HMG-box长度为231 bp,编码77个氨基酸;内蒙古绒山羊SRY基因HMG-box与GenBank中山羊、绵羊、猪、麝香鹿、梅花鹿、牛、水牛和牦牛等偶蹄目动物的核苷酸同源性分别为100.0%,99.1%,86.1%,96.1%,96.1%,97.4%,95.6%和96.9%,推导的氨基酸序列同源性分别为 100.0%,100.0%,83.1%,94.8%,94.8%,94.8%,92.2%和93.5%.基于SRY基因HMG-box核苷酸序列构建的物种间系统进化结果,基本上与这些偶蹄目动物传统的系统分类结果一致.[结论]SRY基因HMG-box区在物种进化中高度保守,有望作为构建系统发育树的候选基因. |
| 关键词: 内蒙古绒山羊 SRY基因；HMG-box；序列分析 |
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| 基金项目:辽宁省教育厅科学研究基金计划项目（2005358） |
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| Molecular characterization of SRY gene HMG-box region in Inner Mongolia cashmere goat |
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| Abstract: |
| 【Objective】 This study aim to clone HMG-box of SRY gene in Inner Mongolia cashmere goat,and analyzed its sequence characteristics.【Method】 From male Inner Mongolia cashmere goat genomic DNA,the fragment of SRY gene containing entire HMG-box region was amplified by PCR with a pair of designed primers based on the sequence of goat SRY gene sequence from GenBank.The amplified fragment was cloned to the pMD18-T vector,and transformed to the bacterium DH5α.The identified positive clone with amplified fragment was sequenced.The sequences homology of SRY gene HMG-box region of some artiodactylas from GenBank was compared with the Inner Mongolia cashmere goat,and based on the sequences of HMG-box region,the phylogenetic tree was constructed by UPGMA method.【Result】 The HMG-box region of Inner Mongolia cashmere goat SRY gene was 231 bp,encoding 77 amino acid residues.The corresponding sequences in GenBank of goat,sheep,pig,musk deer,sika deer,cattle,water buffalo and yak,had homology of 100.0%,99.1%,86.1%,96.1%,96.1%,97.4%,95.6% and 96.9% in nucleotide respectively with the Inner Mongolia cashmere goat,and had homology of 100.0%,100.0%,83.1%,94.8%,94.8%,94.8%,92.2% and 93.5% in induced amino acid respectively with the Inner Mongolia cashmere goat.The clustering based on the sequences of SRY gene HMG-box region among species was generally in agreement with the classic taxonomic relationship.【Conclusion】 The HMG-box of SRY gene is rather conservative in evolution of species,and might be useful in the construction of phylogenetic tree among species. |
| Key words: Inner Mongolia cashmere goat SRY gene HMG-box sequence analysis |
