| 摘要: |
| [目的]研究中国荷斯坦牛趋化因子受体1基因(Chemokine (C-X-C motif) receptor1,CXCR1)编码区的遗传多态性.[方法]采用巢式PCR、DNA测序和创造酶切位点法,对中国荷斯坦牛CXCR1基因编码区下游区域的单核苷酸多态位点(Single nucleotide polymorphisms,SNPs)进行筛查,并对筛查到的SNPs进行群体遗传多态性分析.[结果]发现了819(A/G)、995(A/G)和1008(C/T) 3个SNPs,其中995(A/G)和1008(C/T)为首次报道的多态位点,995(A/G)位点导致第332位的Arg突变为His。CXCR1基因819(A/G)、995(A/G)和1008(C/T) 3个位点在中国荷斯坦牛群体的优势等位基因分别为G、G和C,其等位基因频率分别为0.634 0,0.733 0和0.706 4.经χ2适合性检验,荷斯坦牛在3个位点均未达到Hardy-Weinberg平衡状态(P<0.05);3个位点均表现为中度多态.[结论]中国荷斯坦牛CXCR1基因编码区的遗传多态性比较丰富. |
| 关键词: 中国荷斯坦牛 CXCR1基因 遗传多态性 优势等位基因 |
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| 基金项目:国家自然科学基金重点项目（30230230）；国家科技支撑计划项目（2008BADA4B09） |
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| Genetic polymorphisms within the coding regions of CXCR1 gene in Chinese Holstein cattle |
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| Abstract: |
| 【Objective】 The genetic polymorphism within the coding regions of CXCR1 gene in Chinese Holstein cattle has been detected.【Method】 470 of CXCR1 exon of Chinese Holstein cattle were investigated by nested-PCR,DNA sequencing and CRS-PCR.At the same time,the genetic polymorphism of the group was analyzed.【Result】 Sequencing results showed that three SNPs were identified,namely 819(A/G),995(A/G),1 008(C/T). 819(A/G) and 1 008(C/T) were silent mutations,but 995(A/G) resulted in amino acids alteration Arg332His.In Holstein cattle G and G and C predominant allele were identical at 819(A/G) and 995(A/G) and 1 008(C/T),which were G,G,C and the allelic frequencies were 0.634 0,0.733 0,0.706 4 respectively.χ2 test indicated that all polymorphic sites did not meet Hardy-Weinberg equilibrium (P<0.05) in Chinese Holstein cattle.The value of polymorphism information content indicated that 819(A/G),995(A/G) and 1 008(C/T)s were moderate polymorphism in Chinese Holstein cattle.【Conclusion】 The genetic polymorphism within the coding regions of CXCR1 gene in Chinese Holstein cattle was abundant. |
| Key words: Chinese Holstein cattle CXCR1 gene genetic polymorphism predominant allele |
