| 摘要: |
| [目的]分离和鉴别引起七叶树溃疡病的病原菌,为制定合理的防治技术提供依据.[方法]采用组织分离培养、人工接种、形态观察及核糖体转录间隔区序列(ITS-rDNA)分析,对引起七叶树溃疡病的水渍型溃疡病病原菌NW397和干腐型溃疡病病原菌NW336 2种真菌进行鉴别与比较.[结果]引起七叶树溃疡病的病原为2种不同的真菌,并表现出不同的危害特征.水渍型溃疡病菌NW397 ITS-rDNA序列与多主葡萄座腔菌(Botryosphaeria dothidea Sacc.)等的序列同源性高达99%;干腐型溃疡病菌NW336 ITS-rDNA序列与乌饭树拟茎点菌(Phomopsis vaccinii) 等的同源性高达95%以上.[结论]七叶树水渍型溃疡病菌定名为多主葡萄座腔菌(Botryosphaeria dothidea Sacc.),干腐型溃疡病菌定名为梨干枯拟茎点菌(Phompsis fukushii Tanaka et Mill). |
| 关键词: 七叶树 溃疡病菌 致病性 核糖体转录间隔区序列 |
| DOI： |
| 分类号: |
| 基金项目:科技部自然科技资源共享平台资助项目，陕西省自然科学基金 |
|
| Isolation and identification of two kinds of canker pathogen of Aesculus chinensis |
|
|
| Abstract: |
| 【Objective】 The research studied the isolation and identification of the canker pathogen occurred onAesculus chinensis Beg.【Method】 Traditional Kock’s technology and ITS sequence analysis technology were used to ideatify and compare two canker pathogens:NW397 and NW336.【Result】 Two kinds of canker pathogen ofAesculus chinensis were identified.Pathogen of immerse canker had over 99% homogeneous with fungi Botryosphaeria dothidea Sacc. Pathogen of dry rot canker had over 95% homogeneous with fungi Phomopsis vaccinii,and so on.【Conclusion】 The pathogen of immerse canker (isolate NW397) was identified as Botryosphaeria dothidea Sacc,and the dry rot pathogen (isolate NW336) was identified as Phomopsis fukushii Tanaka et Mill.They were two different pathogen fungi with different disease symptoms. |
| Key words: Aescuus chinensis Beg. canker pathogen pathogensis ITS-rDNA |
