| 摘要: |
| [目的]筛选153活体菌剂的理想填料,以延长153制剂的货架期,提高其大田防病效果.[方法]以固体发酵培养153,分别添加硅藻土、高岭土和非耕作层黄土3种填料,通过活菌量测定和温室防病促生效果观察对理想填料进行筛选.[结果]在3种填料中,以加入非耕作层黄土的活体粉剂的存储时间最长,35 ℃下可以保存70 d以上,活菌量保持水平最高.菌株153对茄子的生长发育有明显促进作用,对植株株高、倒3叶叶面积、干质量及根生长的促进作用最为明显,植株鲜质量的增幅最高可达98.47%,地上部干质量和根干质量的增幅最高分别可达74.86%和82.64%.以非耕作层黄土为填料的菌剂对茄子黄萎病的防效可达82.44%.[结论]通过加入合适的填料,能有效延长菌剂贮存期并提高菌株的生态适应性,为生防菌153从实验室走向生产实践奠定了基础. |
| 关键词: 放线菌 生防菌制剂 菌株153 固态发酵 大丽轮枝菌 |
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| 基金项目:教育部创新团队发展计划项目，西北农林科技大学创新团队计划 |
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| Formulation of biocontrol actinomyces strain 153 and its control effect on Verticillium wilt |
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| Abstract: |
| 【Objective】 The study was done to prolong the shelf life of actinomycetes strain 153 and improve the bio control effect in the field grown condition.【Method】 153 solid-state fermented culture was mixed with diatomite,kaolin as well as non-cultivated loess and screened by the number of living bacteria and their protection effect on eggplant against Verticillium dahliae was studied.【Result】 Non-cultivated loess was better than diatomite,kaolin.With this additive,the biocontrol agent could be stored more than 10 weeks at 35 ℃.The growth and development of eggplant could be promoted obviously by live-cell formulation of 153.Especially,the amplitude of green plant weight attained 98.47%,amplitude of dry weight of stem and dry weight of radix attained 74.86% and 82.64%.The control rate of eggplant Verticillium wilt with the formulation mixed non-cultivated loess reached 82.44%.【Conclusion】By adding suitable additive,the formulation of actinomyces strain 153 can obtain longer shelf life and better environment adaptation. |
| Key words: actinomyce biocontrol agent strain 153 solid fermentation Verticillium dahliae |
