| 摘要: |
| [目的]研究在果实发育过程中嫁接西瓜糖分积累及蔗糖代谢相关酶活性的变化,探讨不同砧木影响果实品质的机理.[方法]采用优质中小型西瓜早佳为接穗和对照,丝瓜、南瓜(铁木真)、葫芦(昌砧力士F1)、野生西瓜1号、野生西瓜2号为砧木,测定嫁接和对照西瓜果实在发育过程中果糖、葡萄糖、蔗糖的含量以及与蔗糖积累相关酶的活性.[结果]与对照相比,野生西瓜2号/早佳组合的果实糖积累极显著增高,而其他嫁接组合的糖积累均降低,丝瓜/早佳组合的果实糖含量最低;自根和嫁接西瓜果实发育过程中蔗糖转化酶(Inv)活性降低,蔗糖磷酸合酶(SPS)活性升高,蔗糖合酶(SS)在西瓜果实发育后期主要起合成作用,促进果实蔗糖积累,蔗糖代谢相关酶净活性值增大.在整个果实发育过程中,Inv、SPS、SS共同作用于嫁接西瓜和自根西瓜果实的糖分积累及构成;嫁接和对照西瓜果实以己糖积累为主,蔗糖积累为辅.[结论]砧木不同,西瓜果实的糖分积累量也不同,其中野生西瓜2号对早佳西瓜品质影响最小,可以作为优良的嫁接砧木在生产实践中应用. |
| 关键词: 砧木 西瓜 糖分积累 蔗糖转化酶 蔗糖磷酸合酶 蔗糖合酶 |
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| 基金项目:国家科技支撑计划 |
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| Influences of rootstocks on the sugar accumulation and activities of sucrose metabolism related enzymes in Citrullus lanatus by grafting |
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| Abstract: |
| 【Objective】 The sugar accumulation,changes of activities of sucrose metabolism related enzymes and the mechanism of the effects on fruit quality by grafting were all studied in this research.【Method】 Zaojia was taken on the CK,and wild watermelon 1 and 2,pumpkin （Tiemuzhen）,calabash （Changzhanlishi F1）and loofah were used as five different rootstocks.Then,fructose,glucose,sucrose and the activities of sucrose metabolism related enzymes were all determinated.【Result】 Compared with the ck,the sugar accumulation increased extremely significant by grafting with the wild watermelon 2,but that of the other treatments all decreased and the sugar accumulation of loofah/Zaojia was the lowest.The sucrose invertase activity decreased;the activitives of sucrose phosphate synthase and net for sucrose metabolism enzymes all increased during the development of own rooted and grafted watermelons.During the whole fruit development including own-rooted and grafted watermelon fruits,the invertase,sucrose phosphate synthase and sucrose synthase together acted on sugar accumulation and constitution,and accumulation of hexose sugar was more than sucrose accumulation in grafted and owned rooted watermelons.【Conclusion】 Different rootstocks had different sugar contents in watermelons,the wild watermelon 2 made the smallest effects on fruit quality,and the sucrose synthase played the important role in promoting sugar accumulation in the latter part of the developing fruits.So it can be used as a good rootstock in the field. |
| Key words: rootstock watermelon sugar accumulation invertase sucrose phosphate synthase sucrose synthase |
