| 摘要: |
| [目的]粒长、粒宽是小麦种子重要的形态性状,该性状对籽粒的外观商品品质、产量及磨粉品质均至关重要,研究不同环境条件下小麦粒长、粒宽的单个标记和复合区间作图的QTL定位.对小麦粒长、粒宽的分子标记辅助选择具有重要参考作用.[方法]应用一个由115个系组成的W7984/Opata 85重组自交系(RIL)群体,建立了由394个DNA分子标记组成的遗传连锁图,在2种不同环境条件下对小麦粒长、粒宽进行了单个标记的回归分析和复合区间作图的QTL定位.[结果]在单个标记的回归分析中检测到5个粒长的QTLs、3个粒宽的QTLs;复合区间作图分析结果表明,控制粒长的QTLs分别位于5BL和7DS上.在5BL上的贡献率为20.20%～20.81%,LOD值为4.50～4.55;在7DS上的贡献率为13.54%～13.91%,LOD值为2.94～3.20.控制粒宽的QTL位于2B上,贡献率为13.71%～19.30%,LOD值为2.98～4.18.[结论]位于5B和7D上的控制粒长的QTL和位于2B上的控制粒宽的QTL在2种条件下均能检测到. |
| 关键词: 粒长 粒宽 重组自交系(RIL) 数量性状基因座位(QTL) 小麦 |
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| 基金项目:西北农林科技大学青年学术骨干支持计划 |
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| Quantitative trait loci analysis for kernel length and width in wheat (Triticum aestivum L.) |
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| Abstract: |
| 【Objective】 Kernel length and width in wheat are very important because of their relationship with yield and milling quality.【Method】 Quantitative trait loci(QTL) analysis for kernel length(LEN) and kernel width(WID) in wheat was conducted using a set of 115 recombinant inbred lines(RILs) derived from a cross between the synthetic hexaploid wheat W7984 and the spring wheat cultivar Opata 85.Synthetic W7984 has larger kernels,especially in length,compared to Opata 85.The stability of these QTLs was evaluated in two environments.【Result】 Corresponding to genome-wide single marker regression analysis(P<0.01),five markers were linked to LEN QTL,three markers were linked to WID QTL in two different environments.Based on composite interval mapping(LOD>2.5),two intervals with contributions of 20.20% to 20.81%,13.54% to 13.91% were found to be linked to long LEN on chromosomes 5BL and 7DS in two different environments.The LOD score was 4.50 to 4.55,2.94 to 3.20.One interval with contributions of 13.71% to 19.30% were found to be linked to wide WID on chromosomes 2BS.The LOD score was 2.98 to 4.18.【Conclusion】 The marker tam72c on 5B,bcd707 on 7D and closest cdo405b to ksuf11a on 2B identified in this study may prove useful for marker assisted selection in LEN,WID and facilitate molecular cloning of this gene for wheat kernel length and width. |
| Key words: kernel length kernel width recombinant inbred line(RIL) quantitative trait loci(QTL) Triticum aestivum L |
