| 摘要: |
| [目的]确定导致大鲵(Andrias davidiamus,giant salamander)腹水病的主要病原菌类型.[方法]从患病大鲵的腹水和肠道分离细菌并进行纯培养,用细菌自动鉴定系统和分子生物学方法对培养细菌进行鉴定,冉对52尾健康的1龄大鲵进行感染试验,以确定病原菌,同时对病原菌进行药物敏感试验.[结果]经过细菌自动鉴定系统确定了4株细菌,室内感染试验确定嗜水气单胞菌(Aeromonas hydrophila)为主要致病菌;细菌16S rDNA基因序列分析也再次证明该菌为嗜水气单胞菌;药物敏感试验显示,该病原菌对庆大霉素、环丙沙星、阿米卡星等药物敏感,对氨卞青霉素、舒巴坦、头孢唑啉等药物具有抗性.[结论]导致大鲵腹水病的主要病原菌为嗜水气单胞菌. |
| 关键词: 大鲵 腹水病 嗜水气单胞菌 |
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| 基金项目:农业科技成果转化基金，国家科技支撑计划，国家科技基础条件平台专项基金，中央级公益性科研院所基本科研业务费专项资金资助项目 |
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| Isolation and identification of the ascitesosis disease pathogen of giant salamander, Andrias davidiamus |
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| Abstract: |
| 【Objective】 The research identified the pathogenic becteria of giant salamander (Andrias davidiamus) that suffered from ascites disease.【Method】 Becteria were isolated from the intestine and ascites of two freshly died giant salamanders.Fifty two Giant salamanders were used to make experimental infection with injection bacterial suspension by intraperitoneal injection.The becteria were identified by the Gram’s staining,VITEK Bacterial Automatic Identification System and sequence analysis of 16S rDNA gene.【Result】 Four different becteria were isolated and finally the becterium 2#FS,which was identified as Aeromonas hydrophila,copied the typical diseased symptoms naturally.So the Aeromonas hydrophilawas the main pathogen of ascitesosis disease pathogen of giant salamander.Sequence analysis of 16S rDNA showed that the main pathogen was Aeromonas hydrophilatoo.The susceptibility test to antibiotics revealed the isolated pathogen was resistant to Ampicillin etc and sensitive to Ciprofloxacin,etc.【Conclusion】 Aeromonas hydrophilawas the pathogenic becteria resulted in ascites disease of gaint salamander. |
| Key words: giant salamander（Andrias davidiamus） Ascitesosis disease Aeromonas hydrophila |
