| 摘要: |
| [目的]研究断奶后不同日龄绵羊(小尾寒羊和滩羊羔羊)小肠pH及其主要消化酶活性的变化规律,为酶制剂和代乳料研制提供依据.[方法]选用健康无病的小尾寒羊和滩羊各36只,在相同条件下饲养,45日龄断奶,分别于80,120,160和200日龄屠宰取样,测定十二指肠、空肠和回肠内容物的pH值及其主要消化酶活性.[结果]绵羊小肠内容物pH值随日龄增长缓慢上升,从偏酸性转变为中性;小尾寒羊和滩羊小肠内容物pH值差异极显著(P<0.05);十二指肠,空肠和回肠3部位内容物pH值间分别存在极显著差异(P<0.01).随着日龄增长,绵羊小肠内容物中乳糖酶活性逐渐降低(P<0.01),并存在品种(P<0.05)和部位(P<0.01)间的差异.绵羊小肠内容物中淀粉酶活性随日龄增长逐渐上升(P<0.01),没有表现出品种之间的差异.但部位间差异极显著(P<0.01).[结论]绵羊内容物中淀粉酶、乳糖酶活性随日龄变化而变化,但pH值较稳定;小肠内容物中pH值和乳糖酶在品种间存在显著差异(P<0.01);十二指肠、空肠和回肠内容物中pH值、乳糖酶和淀粉酶活性在部位间存在极显著差异(P<0.01). |
| 关键词: 小尾寒羊 滩羊 pH值 消化酶 |
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| 基金项目:农业部结构调整重大技术研究专项项目 |
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| Study on pH, digestive enzymes activities between Small-tailed Han sheep and Tan sheep at different days old |
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| Abstract: |
| 【Objective】 The research studied lactase,amylase activities and pH between small-tailed Han sheep and Tan sheep at different ages to provide theoretical basis for developing milk replacer and enzyme preparation. 【Method】 72 healthy sheep in two breeds,Small-tailed Han sheep and Tan sheep were selected in the same condition.They were weaned at 45 days old,and then slaughtered at 80,120,160 and 200 days old for samples.The digesta pH,activities of lactase and amylase in the different segments of the small intestine (duodenum,jejunum, ileum) were measured.【Result】 The results indicated that the pH value in small intestine content slowly increased with the growth,but not significant,from the acid to neutral.There were differences between two breeds （P<0.05）.The pH of the duodenum,jejunum,ileum digesta was significantly different（P<0.01）.These data of lactase in the intestinal digsta decreased with the growth and the two breeds had remarkable differences（P<0.05）.The three parts of intestines exhibited significant differences（P<0.01）.The activities of amylase in the small intestine increased,and no difference was found between the two breeds.But the different segments of the small intestine showed significant differences （P<0.01）.【Conclusion】 The activities of lactase and amylase changed with the growth,but the pH value was stable.The pH value and lactase activities were remarkly different between two breeds（P<0.01）.The pH value,lactase activities and amylase activities in the duodenum,jejunum,ileum digesta were significantly different（P<0.01）. |
| Key words: Small-tailed Han sheep Tan sheep pH digestive enzyme |
