| 摘要: |
| [目的]研究短链和长链饱和脂肪酸,对小鼠前体脂肪细胞增殖分化及脂代谢新因子氧化型低密度脂蛋白受体1(OLR1)转录表达的影响.[方法]分离培养小鼠前体脂肪细胞,用适宜浓度乙酸钠、硬脂酸钠和p38MAPK通路抑制剂处理,通过细胞计数检测处理24和48 h时相关基因mRNA的表达量.[结果]乙酸钠处理组小鼠脂肪细胞数量随处理时间延长显著下降(P<0.05),硬脂酸钠处理组对小鼠细胞数量无显著影响(P>0.05);乙酸钠和硬脂酸钠均显著上调PPARγ2、C/EBPα和OLR1 mRNA的表达(P<0.05);p38MAPK抑制剂能显著抑制PPARγ2和OLR1 mRNA的表达(P<0.05),但对C/EBPα tuRNA表达无显著影响.[结论]乙酸钠可抑制前体脂肪细胞的增殖,但乙酸钠和硬脂酸钠均能促进前体脂肪细胞的分化;OLR1基因在前体脂肪细胞分化过程中通过p38MAPK通路发挥作用,且脂肪酸对前体脂肪细胞分化及OLR1转录表达的影响与p38MAPK通路密切相关. |
| 关键词: 脂肪酸 前体脂肪细胞 |
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| 基金项目:国家自然科学基金，教育部重点科研项目，教育部新世纪优秀人才支持计划，国家科技支撑计划 |
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| Effect of fatty acids on the proliferation and differentiation of mouse adipocytes as well as transcriptional expression of OLR1 |
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| Abstract: |
| 【Objective】 The research studied the effect of short chain and long chain fatty acids on the proliferation and differentiation of mouse preadipocytes,and at the same time,the effect on transcriptional expression of oxidized low-density lipoprotein receptor 1 (OLR1) was also studied.【Method】 Mouse preadipocytes were extracted and cultured,and treated with sodium acetate,sodium stearate as well as p38MAPK inhibitor in suitable concentration.The cell numbers were calculated and related gene mRNA expressions were detected 24 and 48 h after the treatment.【Result】 The result indicated that cell number in sodium acetate treated group decreased significantly with the prolonging of time(P<0.05) while the sodium stearate treat group did not change significantly (P>0.05).Both sodium acetate and sodium stearate significantly up regulated the mRNA expression of PPARγ2,C/EBPα and OLR1(P<0.05).p38MAPK inhibitor could inhibit the mRNA expression of PPARγ2 and OLR1 signifiantly (P<0.05),but had no significant effect on C/EBPα (P>0.05).【Conclusion】 It can be concluded that sodium acetate could inhibit adipocyte proliferation while sodium acetate and sodium stearate could both promote preadipocyte differentiation.OLR1 gene played its role in preadipocyte differentiation process via p38MAPK pathway,moreover,the effect of fatty acids on preadipocyte proliferation and differentiation as well as the transcriptional expression had a close relation with p38MAPK pathway. |
| Key words: fatty acid preadipocyte OLR1 p38MAPK |
